Visualization of protein kinase activities in single cells by antibodies against phosphorylated vimentin and GFAP

被引：21

作者：

Inagaki, N

Tsujimura, K

Tanaka, J

Sekimata, M

Kamei, Y

Inagaki, M

机构：

[1] AICHI CANC CTR, RES INST, IMMUNOL LAB, CHIKUSA KU, NAGOYA, AICHI 464, JAPAN

[2] TOKYO METROPOLITAN INST GERONTOL, DEPT NEUROPHYSIOL, ITABASHI KU, TOKYO 173, JAPAN

来源：

NEUROCHEMICAL RESEARCH | 1996年 / 21卷 / 07期

关键词：

site- and phosphorylation-specific antibody; CaM kinase II; cdc2; kinase;

D O I：

10.1007/BF02532302

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Vimentin and glial fibrillary acidic protein (GFAP) are intermediate filament proteins expressed in the cytoplasm of various types of cells. The head domains of these proteins are phosphorylated by various protein kinases. Site- and phosphorylation-specific antibodies which recognize a phosphorylated serine/threonine residue in the head domains and its flanking sequence provide a useful tool to monitor and visualize protein kinase activities in single cells.

引用

页码：795 / 800

页数：6

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