Construction of heme enzymes: four approaches

被引：35

作者：

Watanabe, Y ^{[1
]}

机构：

[1] Okazaki Natl Res Inst, Ctr Integrated Biosci, Okazaki, Aichi 4448585, Japan

[2] Okazaki Natl Res Inst, Inst Mol Sci, Okazaki, Aichi 4448585, Japan

来源：

CURRENT OPINION IN CHEMICAL BIOLOGY | 2002年 / 6卷 / 02期

关键词：

D O I：

10.1016/S1367-5931(02)00301-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Construction of enzymes that catalyze either desired reactions or exhibit desired substrate specificity is one of the goals of enzymatic study. Rational design of enzymes is an important approach in this field. Another, extremely different, methodology from rational design, directed evolution, has been rapidly developed over the past two years.

引用

页码：208 / 216

页数：9

共 52 条

[1] ROLES OF PROXIMAL LIGAND IN HEME-PROTEINS - REPLACEMENT OF PROXIMAL HISTIDINE OF HUMAN MYOGLOBIN WITH CYSTEINE AND TYROSINE BY SITE-DIRECTED MUTAGENESIS AS MODELS FOR P-450, CHLOROPEROXIDASE, AND CATALASE [J].

ADACHI, S ;

NAGANO, S ;

ISHIMORI, K ;

WATANABE, Y ;

MORISHIMA, I ;

EGAWA, T ;

KITAGAWA, T ;

MAKINO, R .

BIOCHEMISTRY, 1993, 32 (01) :241-252

[2] Directed evolution of biocatalysts [J].

Arnold, FH ;

Volkov, AA .

CURRENT OPINION IN CHEMICAL BIOLOGY, 1999, 3 (01) :54-59

[3] CRYSTALLIZATION AND PRELIMINARY-X-RAY DIFFRACTION ANALYSIS OF P450TERP AND THE HEMOPROTEIN DOMAIN OF P450BM-3, ENZYMES BELONGING TO 2 DISTINCT CLASSES OF THE CYTOCHROME P450 SUPERFAMILY [J].

BODDUPALLI, SS ;

HASEMANN, CA ;

RAVICHANDRAN, KG ;

LU, JY ;

GOLDSMITH, EJ ;

DEISENHOFER, J ;

PETERSON, JA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (12) :5567-5571

[4] Engineering the active site of ascorbate peroxidase [J].

Çelik, A ;

Cullis, PM ;

Sutcliffe, MJ ;

Sangar, R ;

Raven, EL .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 2001, 268 (01) :78-85

[5] Directed evolution of microbial oxidative enzymes [J].

Cherry, JR .

CURRENT OPINION IN BIOTECHNOLOGY, 2000, 11 (03) :250-254

[6] Directed evolution of a fungal peroxidase [J].

Cherry, JR ;

Lamsa, MH ;

Schneider, P ;

Vind, J ;

Svendsen, A ;

Jones, A ;

Pedersen, AH .

NATURE BIOTECHNOLOGY, 1999, 17 (04) :379-384

[7] The structure of nitric oxide synthase oxygenase domain and inhibitor complexes [J].

Crane, BR ;

Arvai, AS ;

Gachhui, R ;

Wu, CQ ;

Ghosh, DK ;

Getzoff, ED ;

Stuehr, DJ ;

Tainer, JA .

SCIENCE, 1997, 278 (5337) :425-431

[8] Hydroxylation of camphor by-reduced oxy-cytochrome P450cam: Mechanistic implications of EPR and ENDOR studies of catalytic intermediates in native and mutant enzymes [J].

Davydov, R ;

Makris, TM ;

Kofman, V ;

Werst, DE ;

Sligar, SG ;

Hoffman, BM .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2001, 123 (07) :1403-1415

[9]

DUNFORD HB, 1999, HEME PEROXIDASES, pCH14

[10] RELATIONSHIP BETWEEN CODING SEQUENCES AND FUNCTION IN HEMOGLOBIN [J].

EATON, WA .

NATURE, 1980, 284 (5752) :183-185

← 1 2 3 4 5 6 →