Gene gun mediated vaccination is superior to manual delivery for immunisation with DNA vaccines expressing protective antigens from Yersinia pestis or Venezuelan Equine Encephalitis virus

被引：52

作者：

Bennett, AM ^{[1
]}

Phillpotts, RJ ^{[1
]}

Perkins, SD ^{[1
]}

Jacobs, SC ^{[1
]}

Williamson, ED ^{[1
]}

机构：

[1] Def Evaluat & Res Agcy, CBD Porton Down, Salisbury SP4 0JQ, Wilts, England

来源：

VACCINE | 1999年 / 18卷 / 7-8期

关键词：

plague; VEE; DNA immunisation;

D O I：

10.1016/S0264-410X(99)00317-5

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Plasmids expressing the V antigen of Yersinia pestis or the E2 glycoprotein of Venezuelan Equine Encephalitis (VEE) virus were used to vaccinate mice by intra-dermal or intra-muscular injection, or by particle-mediated bombardment using the Helios(TM) gene gun. After two immunizations, groups of mice which had received 4 mu g doses of plasmid DNA using the gene gun had IgG levels which were higher than in other groups manually immunised with 12-fold more plasmid DNA. The immunoglobulin isotype profile was predominantly IgG1 following inoculation with either plasmid. Our results indicate that gene gun mediated vaccination can be used to increase the magnitude of the immune response to both bacterial and viral antigens expressed by plasmid DNA. Crown Copyright (C) 1999 Published by Elsevier Science Ltd. All rights reserved.

引用

页码：588 / 596

页数：9

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