Escherichia coli O antigen typing using DNA microarrays

被引：22

作者：

Liu, Yanhong

Fratamico, Pina

机构：

[1] USDA, Agr Res Serv, Eastern Reg Res Ctr, Microbial Food Safety Res Unit, Wyndmoor, PA 19038 USA

[2] USDA, Agr Res Serv, Microbial Biophys & Residue Chem & Core Technol, Wyndmoor, PA 19038 USA

来源：

MOLECULAR AND CELLULAR PROBES | 2006年 / 20卷 / 3-4期

关键词：

DNA microarray; serotyping; Escherichia coli; O7; O104; O111; O157;

D O I：

10.1016/j.mcp.2006.01.001

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

DNA microarrays were developed for rapid identification of different serogroups of Escherichia coli in a single platform. Oligonucleotides, as well as PCR products from genes in the 0 antigen gene clusters of E. coli serogroups 07, 0104, 0111, and O157 were spotted onto glass slides. This was followed by hybridization with labeled long PCR products of the entire 0 antigen gene clusters of these serogroups. Results demonstrated that microarrays consisting of either oligonucleotides or PCR products generated specific signals for each serogroup. This is the first report describing the development of model DNA microarrays for determining the serogroup of E. coli strains. (c) 2006 Elsevier Ltd. All rights reserved.

引用

收藏

页码：239 / 244

页数：6

相关论文

共 28 条

[1]

Al-Khaldi SF, 2002, J AOAC INT, V85, P906

[2] Detection of a Salmonella enterica serovar California strain spreading in Spanish feed mills and genetic characterization with DNA microarrays [J].

Alvarez, J ;

Porwollik, S ;

Laconcha, I ;

Gisakis, V ;

Vivanco, AB ;

Gonzalez, I ;

Echenagusia, S ;

Zabala, N ;

Blackmer, F ;

McClelland, M ;

Rementeria, A ;

Garaizar, J .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2003, 69 (12) :7531-7534

[3] Sequence analysis of the Escherichia coli O15 antigen gene cluster and development of a PCR assay for rapid detection of intestinal and extraintestinal pathogenic E. coli O15 strains [J].

Beutin, L ;

Tao, J ;

Feng, L ;

Krause, G ;

Zimmermann, S ;

Gleier, K ;

Xia, QY ;

Wang, L .

JOURNAL OF CLINICAL MICROBIOLOGY, 2005, 43 (02) :703-710

[4] Selective discrimination of Listeria monocytogenes epidemic strains by a mixed-genome DNA microarray compared to discrimination by pulsed-field gel electrophoresis, ribotyping, and multilocus sequence typing [J].

Borucki, MK ;

Kim, SH ;

Call, DR ;

Smole, SC ;

Pagotto, F .

JOURNAL OF CLINICAL MICROBIOLOGY, 2004, 42 (11) :5270-5276

[5] Discrimination among Listeria monocytogenes isolates using a mixed genome DNA microarray [J].

Borucki, MK ;

Krug, MJ ;

Wayne, WT ;

Call, DR .

VETERINARY MICROBIOLOGY, 2003, 92 (04) :351-362

[6] Expression profiling of the schizont and trophozoite stages of Plasmodium falciparum with a long-oligonucleotide microarray -: art. no. R9 [J].

Bozdech, Z ;

Zhu, JC ;

Joachimiak, MP ;

Cohen, FE ;

Pulliam, B ;

DeRisi, JL .

GENOME BIOLOGY, 2003, 4 (02)

[7] Detection of bacterial pathogens in environmental samples using DNA microarrays [J].

Call, DR ;

Borucki, MK ;

Loge, FJ .

JOURNAL OF MICROBIOLOGICAL METHODS, 2003, 53 (02) :235-243

[8] Microarray analysis of microbial virulence factors [J].

Chizhikov, V ;

Rasooly, A ;

Chumakov, K ;

Levy, DD .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2001, 67 (07) :3258-3263

[9] The impact of genomics on drug discovery [J].

Debouck, C ;

Metcalf, B .

ANNUAL REVIEW OF PHARMACOLOGY AND TOXICOLOGY, 2000, 40 :193-207

[10] Development of PCR assays targeting genes in O-antigen gene clusters for detection and identification of Escherichia coli O45 and O55 serogroups [J].

DebRoy, C ;

Fratamico, PM ;

Roberts, E ;

Davis, MA ;

Liu, YH .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2005, 71 (08) :4919-4924