The expression of PADI4 in synovium of rheumatoid arthritis

被引：31

作者：

Chang, Xiaotian ^{[1
]}

Zhao, Yan ^{[1
]}

Sun, Shui ^{[2
]}

Zhang, Yunzhong ^{[3
]}

Zhu, Youming ^{[1
]}

机构：

[1] Shandong Acad Med Sci, Key Lab Biodrugs, Minist Hlth, Med Biotechnol Res Ctr, Jinan 250062, Shandong, Peoples R China

[2] Prov Hosp Shandong, Dept Arthrosurg, Jinan, Shandong, Peoples R China

[3] Shandong Hosp Labor Hlth & Occupat Dis Control, Dept Rheumatol, Jinan, Shandong, Peoples R China

来源：

RHEUMATOLOGY INTERNATIONAL | 2009年 / 29卷 / 12期

基金：

中国国家自然科学基金;

关键词：

Rheumatoid arthritis; PADI4; Synovial fluid; Synovial membrane; RF; Anti-CCP; CITRULLINATED PEPTIDE ANTIBODIES; PEPTIDYLARGININE DEIMINASE-4; DISEASE; ASSOCIATION; PROTEINS;

D O I：

10.1007/s00296-009-0870-2

中图分类号：

R5 [内科学];

学科分类号：

100201 [内科学];

摘要：

PADI4 that catalyzes the conversion of peptidylarginine to citrulline is associated with rheumatoid arthritis in some populations. The current study investigated the expressions of PADI4 in synovial fluid of RA (n = 73), osteoarthritis (OA, n = 96) and ankylosing spondylitis (AS, n = 32) using ELISA and western blotting following immuno-precipitation (n = 6 for each diseases). The study also compared the mRNA level of PADI4 in the synovial membrane of RA with the levels in the samples of OA and AS (n = 6 for each diseases) using real time PCR. ELISA detected a higher level of PADI4 in SF of RA than in samples of OA and AS (P = 0.0001). The level of PADI4 was significantly correlated with the level of rheumatic factor (P = 0.015), but not with anti cyclic citrullinated peptide antibody (anti-CCP) in the RA fluids. Western blotting confirmed the expression of PADI4 in SF of RA. Quantitative PCR measured higher transcription of PADI4 in the synovial membrane of RA than in the samples of OA and AS. The results confirmed increased expression of PADI4 in synovium of RA.

引用

页码：1411 / 1416

页数：6

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