Functional scFv antibody sequences against the organophosphorus pesticide chlorpyrifos

被引：9

作者：

Alcocer, MJC ^{[1
]}

Doyen, C ^{[1
]}

Lee, HA ^{[1
]}

Morgan, MRA ^{[1
]}

机构：

[1] Inst Food Res, Norwich NR4 7UA, Norfolk, England

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2000年 / 48卷 / 02期

关键词：

variable region; antibody engineering; immunoassay; recombinant antibody; antibody pseudogene;

D O I：

10.1021/jf990690u

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Two functional single-chain Fv (scFv) antibodies that recognize specifically the widely used organophosphorus pesticide chlorpyrifos-ethyl were derived from two murine hybridoma cell lines. It is shown that the functional scFvs could be isolated without any rounds of selection, with a success rate dependent on the efficiency of amplification of the functional light chain gene family by the specific primers. Besides four new functional immunoglobulin variable gene sequences, the isolation of a new pseudogene is reported.

引用

页码：335 / 337

页数：3

共 16 条

[11] Development of a magnetic particle-based enzyme immunoassay for the quantitation of chlorpyrifos in water [J].