Comparing the distribution of RAPD and RFLP markers in a high density linkage map of sugar beet

被引：31

作者：

Nilsson, NO ^{[1
]}

Hallden, C ^{[1
]}

Hansen, M ^{[1
]}

Hjerdin, A ^{[1
]}

Sall, T ^{[1
]}

机构：

[1] LUND UNIV, DEPT GENET, S-22362 LUND, SWEDEN

来源：

GENOME | 1997年 / 40卷 / 05期

关键词：

sugar beet; linkage map; RAPD; RFLP; clustering;

D O I：

10.1139/g97-085

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The distribution of RAPD markers was compared with that of RFLP markers in a high density linkage map of sugar beet. The same mapping population of 161 F-2 individuals was used to generate all the marker data. The total map comprises 160 RAPD and 248 RFLP markers covering 508 cM. Both the RAPD and the RFLP markers show a high degree of clustering over the nine linkage groups. The pattern is compatible with a strong distal localization of recombination in the sugar beet. It leads generally to one major cluster of markers in the centre of each linkage group. In regions of high marker density, dominant RAPD markers present in either linkage phase and codominant RFLP markers are subclustered relative to each other. This phenomenon is shown to be attributable to: (i) effects of the mapping procedure when dominant and codominant data are combined, (ii) effects of the mapping procedure when dominant data in both linkage phases are combined, and (iii) genuine differences in the way RAPD and RFLP markers are recruited.

引用

页码：644 / 651

页数：8

共 34 条

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