Nucleotide sequence of the blaRTG-2 (CARB-5) gene and phylogeny of a new group of carbenicillinases

被引：23

作者：

Choury, D

Szajnert, MF

Joly-Guillou, ML

Azibi, K

Delpech, M

Paul, G

机构：

[1] UFR Cochin Port Royal, Lab Biol Mol Cellules Eucaryotes, F-75014 Paris, France

[2] UFR Cochin Port Royal, INSERM U129, F-75014 Paris, France

[3] UFR Cochin Port Royal, Lab Rech Microbiol, F-75014 Paris, France

[4] UFR Xavier Bichat, F-75018 Paris, France

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2000年 / 44卷 / 04期

关键词：

D O I：

10.1128/AAC.44.4.1070-1074.2000

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We determined the nucleotide sequence of the bla gene for the Acinetobacter calcoaceticus beta-lactamase previously described as CARB-5. Alignment of the deduced amino acid sequence with those of known beta-lactamases revealed that CARB-5 possesses an RTG triad in box VII, as described for the Proteus mirabilis GN79 enzyme, instead of the RSG consensus characteristic of the other carbenicillinases. Phylogenetic studies showed that these RTG enzymes constitute a new, separate group, possibly ancestors of the carbenicillinase family.

引用

收藏

页码：1070 / 1074

页数：5

相关论文

共 27 条

[1] Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].

Altschul, SF ;

Madden, TL ;

Schaffer, AA ;

Zhang, JH ;

Zhang, Z ;

Miller, W ;

Lipman, DJ .

NUCLEIC ACIDS RESEARCH, 1997, 25 (17) :3389-3402

[2]

BOISSINOT M, 1990, J BIOL CHEM, V265, P1225

[3] A FUNCTIONAL CLASSIFICATION SCHEME FOR BETA-LACTAMASES AND ITS CORRELATION WITH MOLECULAR-STRUCTURE [J].

BUSH, K ;

JACOBY, GA ;

MEDEIROS, AA .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1995, 39 (06) :1211-1233

[4] THE PHOTOTROPHIC BACTERIUM RHODOPSEUDOMONAS-CAPSULATA SP108 ENCODES AN INDIGENOUS CLASS-A BETA-LACTAMASE [J].

CAMPBELL, JIA ;

SCAHILL, S ;

GIBSON, T ;

AMBLER, RP .

BIOCHEMICAL JOURNAL, 1989, 260 (03) :803-812

[5] Characterization and nucleotide sequence of CARB-6, a new carbenicillin-hydrolyzing β-lactamase from Vibrio cholerae [J].

Choury, D ;

Aubert, G ;

Szajnert, MF ;

Azibi, K ;

Delpech, M ;

Paul, G .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1999, 43 (02) :297-301

[6] PHYLOGENY OF LCR-1 AND OXA-5 WITH CLASS-A AND CLASS-D BETA-LACTAMASES [J].

COUTURE, F ;

LACHAPELLE, J ;

LEVESQUE, RC .

MOLECULAR MICROBIOLOGY, 1992, 6 (12) :1693-1705

[7] NUCLEOTIDE-SEQUENCE OF SHV-2 BETA-LACTAMASE GENE [J].

GARBARGCHENON, A ;

GODARD, V ;

LABIA, R ;

NICOLAS, JC .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1990, 34 (07) :1444-1446

[8] ACQUISITION BY ESCHERICHIA-COLI OF PLASMID-BORNE BETA-LACTAMASES NORMALLY CONFINED TO PSEUDOMONAS SPP [J].

HEDGES, RW ;

MATTHEW, M .

PLASMID, 1979, 2 (02) :269-278

[9] REFINED CRYSTAL-STRUCTURE OF BETA-LACTAMASE FROM STAPHYLOCOCCUS-AUREUS PC1 AT 2.0-A RESOLUTION [J].

HERZBERG, O .

JOURNAL OF MOLECULAR BIOLOGY, 1991, 217 (04) :701-719

[10] NUCLEOTIDE-SEQUENCE AND PHYLOGENY OF SHV-2 BETA-LACTAMASE [J].

HULETSKY, A ;

COUTURE, F ;

LEVESQUE, RC .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1990, 34 (09) :1725-1732