Localization of 113 anchor loci in pigs: improvement of the comparative map for humans, pigs, and goats

被引：86

作者：

Pinton, P

Schibler, L

Cribiu, E

Gellin, J

Yerle, M

机构：

[1] INRA, Dept Genet Anim, Lab Genet Cellulaire, F-31326 Castanet Tolosan, France

[2] Lab Genet Biochim & Cytogenet, F-78350 Jouy En Josas, France

来源：

MAMMALIAN GENOME | 2000年 / 11卷 / 04期

关键词：

D O I：

10.1007/s003350010057

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In total, 113 genes that have already been located in humans and goats were cytogenetically mapped in pigs. For this purpose, 165 gene-containing bacterial artificial chromosomes (BACs) isolated in goats were used in heterologous fluorescent in situ hybridization on porcine chromosomes. Among them, 113 (or 69%) gave clear and specific signals, and 52 did not work in heterologous conditions. These localizations are a significant contribution to development of the porcine gene map and also to the comparative map for humans and pigs. They allowed us to specify the information obtained by Zoo-FISH while taking the gene order into account; the number of conserved fragments detected for human and pig chromosomes reached 84. The average size of conserved fragments could be estimated at 33 cM. As these genes had already been mapped in goats, the comparison was extended to ruminants. The previous results obtained in this species, suggesting a correlation between human chromosome abnormalities and evolutionary breakpoints, were confirmed in pigs.

引用

页码：306 / 315

页数：10

共 26 条

[1] Novel conserved synteny between human chromosome 22 and cattle chromosome 22 established by linkage mapping of transducin alpha-1 subunit (GNAZ) [J].

Aleyasin, A ;

Barendse, W .

MAMMALIAN GENOME, 1997, 8 (06) :458-459

[2] Comparative genome organization of vertebrates [J].

Andersson, L ;

Archibald, A ;

Ashburner, M ;

Audun, S ;

Barendse, W ;

Bitgood, J ;

Bottema, C ;

Broad, T ;

Brown, S ;

Burt, D ;

Charlier, C ;

Copeland, N ;

Davis, S ;

Davisson, M ;

Edwards, J ;

Eggen, A ;

Elgar, G ;

Eppig, JT ;

Franklin, I ;

Grewe, P ;

Gill, T ;

Graves, JAM ;

Hawken, R ;

Hetzel, J ;

Hilyard, A ;

Jacob, H ;

Jaswinska, L ;

Jenkins, N ;

Kunz, H ;

Levan, G ;

Lie, O ;

Lyons, L ;

Maccarone, P ;

Mellersh, C ;

Montgomery, G ;

Moore, S ;

Moran, C ;

Morizot, D ;

Neff, M ;

Nicholas, F ;

OBrien, S ;

Parsons, Y ;

Peters, J ;

Postlethwait, J ;

Raymond, M ;

Rothschild, M ;

Schook, L ;

Sugimoto, Y ;

Szpirer, C ;

Tate, M .

MAMMALIAN GENOME, 1996, 7 (10) :717-734

[3] A medium-density genetic linkage map of the bovine genome [J].

Barendse, W ;

Vaiman, D ;

Kemp, SJ ;

Sugimoto, Y ;

Armitage, SM ;

Williams, JL ;

Sun, HS ;

Eggen, A ;

Agaba, M ;

Aleyasin, SA ;

Band, M ;

Bishop, MD ;

Buitkamp, J ;

Byrne, K ;

Collins, F ;

Cooper, L ;

Coppettiers, W ;

Denys, B ;

Drinkwater, RD ;

Easterday, K ;

Elduque, C ;

Ennis, S ;

Erhardt, G ;

Ferretti, L ;

Flavin, N ;

Gao, Q ;

Georges, M ;

Gurung, R ;

Harlizius, B ;

Hawkins, G ;

Hetzel, J ;

Hirano, T ;

Hulme, D ;

Jorgensen, C ;

Kessler, M ;

Kirkpatrick, BW ;

Konfortov, B ;

Kostia, S ;

Kuhn, C ;

Lenstra, JA ;

Leveziel, H ;

Lewin, HA ;

Leyhe, B ;

Lil, L ;

Burriel, IM ;

McGraw, RA ;

Miller, JR ;

Moody, DE ;

Moore, SS ;

Nakane, S .

MAMMALIAN GENOME, 1997, 8 (01) :21-28

[4] Zooming in on the human-mouse comparative map: Genome conservation re-examined on a high-resolution scale [J].

Carver, EA ;

Stubbs, L .

GENOME RESEARCH, 1997, 7 (12) :1123-1137

[5] Emerging patterns of comparative genome organization in some mammalian species as revealed by Zoo-FISH [J].

Chowdhary, BP ;

Raudsepp, T ;

Frönicke, L ;

Scherthan, H .

GENOME RESEARCH, 1998, 8 (06) :577-589

[6] Expansion of the pig comparative map by expressed sequence tags (EST) mapping [J].

Fridolfsson, AK ;

Hori, T ;

Wintero, AK ;

Fredholm, M ;

Yerle, M ;

Robic, A ;

Andersson, L ;

Ellegren, H .

MAMMALIAN GENOME, 1997, 8 (12) :907-912

[7] Human and porcine correspondence of chromosome segments using bidirectional chromosome painting [J].

Goureau, A ;

Yerle, M ;

Schmitz, A ;

Riquet, J ;

Milan, D ;

Pinton, P ;

Frelat, G ;

Gellin, J .

GENOMICS, 1996, 36 (02) :252-262

[8] Mapping of 22 expressed sequence tags isolated from a porcine small intestine cDNA library [J].

Jorgensen, CB ;

Wintero, AK ;

Yerle, M ;

Fredholm, M .

MAMMALIAN GENOME, 1997, 8 (06) :423-427

[9] A successful strategy for comparative mapping with human ESTs: 65 new regional assignments in the pig [J].

Lahbib-Mansais, Y ;

Dalias, G ;

Milan, D ;

Yerle, M ;

Robic, A ;

Gyapay, G ;

Gellin, J .

MAMMALIAN GENOME, 1999, 10 (02) :145-153

[10] LOCALIZATION OF IGF1R AND EDN GENES TO PIG CHROMOSOME-1 AND CHROMOSOME-7 BY IN-SITU HYBRIDIZATION [J].

LAHBIBMANSAIS, Y ;

YERLE, M ;

GELLIN, J .

CYTOGENETICS AND CELL GENETICS, 1995, 71 (03) :225-227

← 1 2 3 →