Impact of long-term application of industrial wastewater on the emergence of resistance traits in Azotobacter chroococcum isolated from rhizospheric soil

被引:111
作者
Aleem, A [1 ]
Isar, J [1 ]
Malik, A [1 ]
机构
[1] Aligarh Muslim Univ, Fac Agr Sci, Dept Agr Microbiol, Aligarh 202002, Uttar Pradesh, India
关键词
industrial wastewater; soil; metal resistance; antibiotic resistance; plasmid DNA; Azotobacter chroococcum;
D O I
10.1016/S0960-8524(02)00134-7
中图分类号
S2 [农业工程];
学科分类号
0828 ;
摘要
A total of 57 (36 and 21) Azotobacter chroococcum were isolated from wheat (Triticum aestivum) rhizospheric soil irrigated with industrial wastewater (about a decade) and ground water (uncontaminated) and characterized on the basis of morphological, cultural and biochemical characteristics. Rhizospheric soils were analyzed for metal concentrations by atomic absorption spectrophotometery and the test soil samples were contaminated with Fe, Zn, Cu, Cr, Ni and Pb. All the isolates of A. chroococcum were tested for their resistance against Hg2+, Cd2+, CU2+, Cr3+, Cr6+, Zn2+, Ni2+ and Pb2+. Among 36 isolates of Azotobacter from soil irrigated with industrial wastewater, 94.4% were resistant to Pb2+ and Hg2+ and 86.1%, 77.5% and 63.8% were resistant to Zn2+, Cr6+ and Cr3+ respectively. The highest minimum inhibitory concentration of 200 mug/ml for Hg2+ and 1600 mug/ml for other metals were observed against these bacteria from soil. The incidences of metal resistance and MICs of metals for A. chroococcum from wastewater irrigated soil were significantly different to those of uncontaminated soil. All A. chroococcum isolates were tested for their resistance against I I commonly used antibiotics/drugs. 91.6% were found to be resistant against nitrofurantoin while 86.40%, and 80.5% were found to be resistant against polymyxin-B and co-trimoxazole respectively. Agarose gel electrophoresis using the miniprep method for plasmid isolation revealed that these isolates harboured plasmids of molecular weights 58.8 and 64.5 kb using EcoRI and HindIII digests of lambda DNA and undigested lambda DNA as standard markers. (C) 2002 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:7 / 13
页数:7
相关论文
共 39 条
[1]  
[Anonymous], 1999, INDIAN J ENVIRON HEA
[2]   Multiple-metal tolerance in Pseudomonas fluorescens and its biotechnological significance [J].
Appanna, VD ;
Gazso, LG ;
StPierre, M .
JOURNAL OF BIOTECHNOLOGY, 1996, 52 (02) :75-80
[4]  
BABAK N. M., 1966, MIKROBIOLOGIYA, V35, P162
[5]  
Bansal O. P., 1998, Indian Journal of Environmental Health, V40, P51
[6]  
BAUER AW, 1966, AM J CLIN PATHOL, V45, P493
[7]   VARIATIONS IN THE RESPONSE OF SALT-STRESSED RHIZOBIUM STRAINS TO BETAINES [J].
BERNARD, T ;
POCARD, JA ;
PERROUD, B ;
LERUDULIER, D .
ARCHIVES OF MICROBIOLOGY, 1986, 143 (04) :359-364
[8]  
BHAGAT R, 1991, INT GOLD JUB S IND S
[9]   SPECIES VARIATION AND PLASMID INCIDENCE AMONG FLUORESCENT PSEUDOMONAS STRAINS ISOLATED FROM AGRICULTURAL AND INDUSTRIAL SOILS [J].
CAMPBELL, JIA ;
JACOBSEN, CS ;
SORENSEN, J .
FEMS MICROBIOLOGY ECOLOGY, 1995, 18 (01) :51-62
[10]   Sequential fractionation and plant availability of heavy metals as affected by sewage sludge applications to soil [J].
Canet, R ;
Pomares, F ;
Tarazona, F ;
Estela, M .
COMMUNICATIONS IN SOIL SCIENCE AND PLANT ANALYSIS, 1998, 29 (5-6) :697-716