Endophytic fungal DNA, the source of contamination in spruce needle DNA

被引：31

作者：

Camacho, FJ

Gernandt, DS

Liston, A

Stone, JK

Klein, AS

机构：

[1] UNIV NEW HAMPSHIRE, GENET PROGRAM, DURHAM, NH 03824 USA

[2] UNIV NEW HAMPSHIRE, DEPT BIOCHEM & MOL BIOL, DURHAM, NH 03824 USA

来源：

MOLECULAR ECOLOGY | 1997年 / 6卷 / 10期

关键词：

fungal endophytes; DNA contamination; Hormonema dematioides; Aureobasidium pullulans; Rhizosphaera kalkhoffii; Picea;

D O I：

10.1046/j.1365-294X.1997.00266.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

DNA isolated and amplified from higher plants may originate from symbiotic microbes occupying plant tissues. A recent report on the phylogeny of Picea contained sequence data that upon later analysis proved to originate from filamentous ascomycetes. Isolates of endophytic fungi from Picea foliage collected from the same location as the original samples were examined to identify the source of the contaminating DNA. The ITS region of isolates was screened by Southern blotting using an oligonucleotide probe homologous to a unique portion of the reported 'spruce' sequences. This study identifies a DNA sequence originally attributed to Picea engelmannii (Engelmann spruce) as Hormonema dematioides, a ubiquitous foliar endophyte of conifers. Infections of plants by endophytic fungi are common and their presence is not revealed by external symptoms. Plant molecular researchers should be aware of the potential for this type of DNA contamination.

引用

页码：983 / 987

页数：5

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