Galactomannan hydrolysis and mannose metabolism in Cellvibrio mixtus

被引：22

作者：

Centeno, Maria S. J.

Guerreiro, Catarina I. P. D.

Dias, Fernando M. V.

Morland, Carl

Tailford, Louise E.

Goyal, Arun

Prates, Jose A. M.

Ferreira, Luis M. A.

Caldeira, Rui M. H.

Mongodin, Emmanuel F.

Nelson, Karen E.

Gilbert, Harry J.

Fontes, Carlos M. G. A.

机构：

[1] Polo Univ Alto Ajuda, Fac Vet Med, CIISA, P-1300477 Lisbon, Portugal

[2] Newcastle Univ, Sch Med, Inst Cell & Mol Biosci, Newcastle Upon Tyne NE1 7RU, Tyne & Wear, England

[3] Inst Genom Res, Rockville, MD USA

来源：

FEMS MICROBIOLOGY LETTERS | 2006年 / 261卷 / 01期

关键词：

alpha-galactosidase; epimerase; galactomannan; glycoside hydrolase; Cellvibrio; GLYCOSIDE HYDROLASE FAMILY-5; FLUORESCENS SUBSP CELLULOSA; ALPHA-GALACTOSIDASE; MODULAR ARCHITECTURE; CRYSTAL-STRUCTURE; XYLANASES; ENZYMES; CLONING;

D O I：

10.1111/j.1574-6968.2006.00342.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Galactomannan hydrolysis results from the concerted action of microbial endo-mannanases, manosidases and alpha-galactosidases and is a mechanism of intrinsic biological importance. Here we report the identification of a gene cluster in the aerobic soil bacterium Cellvibrio mixtus encoding enzymes involved in the degradation of this polymeric substrate. The family 27 alpha-galactosidase, termed CmAga27A, preferentially hydrolyse galactose containing polysaccharides. In addition, we have characterized an enzyme with epimerase activity, which might be responsible for the conversion of mannose into glucose. The role of the identified enzymes in the hydrolysis of galactomannan by aerobic bacteria is discussed.

引用

页码：123 / 132

页数：10

共 26 条

[1] ISOLATION OF HIGH MOLECULAR WEIGHT DNA FROM HEMOPHILUS INFLUENZAE [J].