Mass spectrometric analysis of benzoylated sialooligosaccharides and differentiation of terminal α2→3 and α2→6 sialogalactosylated linkages at subpicomole levels

被引：34

作者：

Chen, P ^{[1
]}

Werner-Zwanziger, U ^{[1
]}

Wiesler, D ^{[1
]}

Pagel, M ^{[1
]}

Novotny, MV ^{[1
]}

机构：

[1] Indiana Univ, Dept Chem, Bloomington, IN 47405 USA

来源：

ANALYTICAL CHEMISTRY | 1999年 / 71卷 / 21期

关键词：

D O I：

10.1021/ac990674w

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Perbenzoylated sialooligosaccharides were found to be stable derivatives, giving intense signals during the matrix-assisted laser desorption/ionization (MALDI) mass spectrometric analysis in the positive-ion mode. Terminal Neu5NAc alpha 2-->3 and alpha 2-->6Gal units of oligosaccharides undergo characteristic structural changes during benzoylation, yielding easily recognizable mass spectral patterns. Subpicomole carbohydrate samples were successfully benzoylated and analyzed through MALDI mass spectrometry.

引用

页码：4969 / 4973

页数：5

共 24 条

[1] POLYOMA-VIRUS ADSORBS TO SPECIFIC SIALYLOLIGOSACCHARIDE RECEPTORS ON ERYTHROCYTES [J].