Mechanisms of peroxidase oxidation of o-dianisidine, 3,3′,5,5′-tetramethylbenzidine, and o-phenylenediamine in the presence of sodium dodecyl sulfate

被引：29

作者：

Kireyko, AV ^{[1
]}

Veselova, IA ^{[1
]}

Shekhovtsova, TN ^{[1
]}

机构：

[1] Moscow MV Lomonosov State Univ, Fac Chem, Moscow 119992, Russia

来源：

RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY | 2006年 / 32卷 / 01期

基金：

俄罗斯基础研究基金会;

关键词：

o-dianisidine; horseradish peroxidase; oxidation mechanism; o-phenylenediamine; sodium dodecyl; sulfate; 3,3 ',5,5 '-tetramethylbenzidine;

D O I：

10.1134/S1068162006010079

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Peroxidase oxidation of o-dianisidine, 3,3',5,5'-tetramethylbenzidine, and o-phenylenediamine in the presence of sodium dodecyl sulfate (SIDS), an anionic surfactant, was spectrophotometrically studied. It was found that 0.1-100 mM SDS concentrations stabilize intermediates formed in the peroxidase oxidation of these substrates. The cause of the stabilization is an electrostatic interaction between positively charged intermediates and negatively charged surfactant.

引用

页码：71 / 77

页数：7

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