Interaction between ellagic acid and calf thymus DNA studied with flow linear dichroism UV-VIS spectroscopy

被引：36

作者：

Thulstrup, PW ^{[1
]}

Thormann, T ^{[1
]}

Spanget-Larsen, J ^{[1
]}

Bisgaard, HC ^{[1
]}

机构：

[1] Roskilde Univ, Inst Life Sci & Chem, Dept Life Sci & Chem, DK-4000 Roskilde, Denmark

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1999年 / 265卷 / 02期

关键词：

D O I：

10.1006/bbrc.1999.1694

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The interaction between ellagic acid and DNA has been characterized with respect to the geometry of the ellagic acid-DNA complex, and the active form of ellagic acid has been identified. Optical spectroscopic methods have been employed to examine the interaction between double-stranded calf thymus DNA and ellagic acid in low-ionic-strength aqueous solutions at pH values of 5.5, 7.0, and 8.8. Based on normal absorption titration and how linear dichroism experiments, it is confirmed that the neutral form of ellagic acid present at pH 5.5 binds to double-stranded DNA. It is found that the plane of the ellagic acid chromophore is positioned at an angle relative to the DNA helix axis, which is in accordance with intercalation of ellagic acid in DNA. It is concluded that at higher values of pH no or a very limited amount of ellagic acid binds to DNA. These results prove that the direct interaction between ellagic acid and DNA must be taken into account when evaluating the mechanism underlying the observed biological effects of this plant phenol. (C) 1999 Academic Press.

引用

页码：416 / 421

页数：6

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