Expression and characteristics of the gene encoding azoreductase from Rhodobacter sphaeroides AS1.1737

被引：85

作者：

Bin, Y ^{[1
]}

Zhou, ZT

Jing, W

Du, CH

Hou, HM

Song, ZY

Bao, YM

机构：

[1] Dalian Univ Technol, Sch Environm & Biol Sci & Technol, Dalian 116023, Peoples R China

[2] Dalian Inst Light Ind, Dalian 116034, Peoples R China

来源：

FEMS MICROBIOLOGY LETTERS | 2004年 / 236卷 / 01期

关键词：

azoreductase; biodegradarion; azo dyes; pGEX4T-1;

D O I：

10.1016/j.femsle.2004.05.034

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A gene that encodes a protein with azoreductase activity was obtained by PCR amplification from Rhodobacter sphaeroides AS1.1737. The enzyme, with a molecular weight of 18.7 kD, was heterologously expressed in Escherichia coli and its azoreductase activity was characterized. Furthermore, the reduction mechanism of azo dyes catalyzed by the azoreductase was studied in detail. The presence of a hydrazo-intermediate was identified, which provided a convincing evidence for the assumption that azo dyes were degraded via an incomplete reduction stage. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：129 / 136

页数：8

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