High-resolution X-ray microtomography for the detection of lung tumors in living mice

被引:69
作者
De Clerck, NM
Meurrens, K
Weiler, H
Van Dyck, D
Vanhoutte, G
Terpstra, P
Postnov, AA
机构
[1] Univ Antwerp, Dept Biomed Sci, RUCA, B-2020 Antwerp, Belgium
[2] PHILIP MORRIS Res Labs, B-3001 Grauwmeer, Belgium
[3] PHILIP MORRIS Res Labs, D-51149 Cologne, Germany
[4] Univ Antwerp, Dept Phys, B-2020 Antwerp, Belgium
来源
NEOPLASIA | 2004年 / 6卷 / 04期
关键词
X-ray microtomography; in vivo scanning; lung tumors; 3D rendering; urethane;
D O I
10.1593/neo.03481
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
In the present study, the feasibility of applying high-resolution microtomography (micro-CT) for the detection of lung tumors was investigated in live mice at an early and more advanced stage of tumor development. The chest area of anesthesized mice was scanned by X-ray micro-CT. In mice with a minor and heavy tumor load, micro-CT proved to be a fast and noninvasive imaging device for the detection of lung tumors. After validation of the CT data by histologic sectioning, it was shown that the majority of tumors could be distinguished in the reconstructed virtual slices obtained by micro-CT. The data from micro-CT were also confirmed by visual inspection of the inflated and excised lungs postmortem. In vivo micro-CT opens broad perspectives for imaging tumor development and its progression in a noninvasive way. Micro-CT also allows for longitudinal evaluation of the treatment of lung cancer by drugs.
引用
收藏
页码:374 / 379
页数:6
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