Translation of NRF mRNA is mediated by highly efficient internal ribosome entry

被引：56

作者：

Oumard, A ^{[1
]}

Hennecke, M ^{[1
]}

Hauser, H ^{[1
]}

Nourbakhsh, M ^{[1
]}

机构：

[1] GBF Natl Res Inst Biotechnol, Dept Gene Regulat & Differentiat, D-38124 Braunschweig, Germany

来源：

MOLECULAR AND CELLULAR BIOLOGY | 2000年 / 20卷 / 08期

关键词：

D O I：

10.1128/MCB.20.8.2755-2759.2000

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The ubiquitous transcription factor NRF (NF-kappa B repressing factor) is a constitutive transcriptional silencer of the multifunctional cytokine interferon-beta. NRF mRNA contains a Long 5' untranslated region (5'UTR) predicted to fold into a strong secondary structure. The presence of stable hairpins is known to be incompatible with efficient translation by ribosomal scanning. Using dicistronic reporter gene constructs, we show that the NRF 5'UTR acts as an internal ribosome entry site (IRES) which directs ribosomes to the downstream start codon by a cap-independent mechanism. The relative activity of this IRES in various cell lines is at least 30-fold higher than that of picornaviral IRESs. The NRF 5'UTR also functions as a translational enhancer in the context of monocistronic mRNAs. Our results indicate that the NRF 5'UTR contains a highly potent IRES, which may allow for an alternate mode of translation under physiological conditions in which cap-dependent translation is inhibited.

引用

页码：2755 / 2759

页数：5

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