Effect of monensin on secretory granules and basal beta-endorphin secretion in the melanotroph of the rat pituitary

The effect of monensin on the Golgi complex, formation of secretory granules and basal P-endorphin secretion in cultured melanotrophs from the rat pituitary was studied. Earlier studies on the effect of monensin on regulated secretion have generally showed only minor effects on secretory granules. The initial (within 5 min) effect of monensin on the melanotroph was the appearance of large vacuoles at the trans-side of the thiamine pyrophosphatase-positive trans-most Golgi cisternae. This was associated with a dose-dependent inhibition of the condensation of electron-dense secretory products. After 1 h of treatment with 1 mu M monensin the Golgi stack was completely vacuolized. At the same time mature secretory granules were enlarged to severalfold their original size, and after 4 h of treatment secretory granules were no longer observed. Despite the marked effects on granule formation and mature secretory granules monensin did not affect the basal release of beta-endorphin-immunoreactive material during continued incubation for up to 4 h, indicating that basal peptide secretion can bypass the monensin block.