Polyrotaxane approach for synthesis of continuous beds for capillary electrochromatography

被引:24
作者
Kornysova, O
Surna, R
Snitka, V
Pyell, U
Maruska, A
机构
[1] Vytautas Magnus Univ, Dept Chem, LT-3035 Kaunas, Lithuania
[2] Univ Marburg, Dept Chem, D-35032 Marburg, Germany
[3] Kaunas Univ Technol, Sci Ctr Microsyst & Nanotechnol, LT-3031 Kaunas, Lithuania
关键词
continuous bed; electrochromatography; stationary phases; enantiomer separation; rotaxanes;
D O I
10.1016/S0021-9673(02)00824-5
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The polyrotaxane formation approach was evaluated for synthesis of continuous beds for capillary electrochromatography. This approach has the advantage of generating diverse electroosmotic and chromatographic properties without chemical reactions. The polyrotaxane derivatized continuous beds were formed adding the macrocyclic compounds to the solution of neutral acrylic monomers and crosslinker prior to the initiation of the polymerisation. Cationic and anionic derivatives of beta-cyclodextrin were used as macrocyclic compounds. Investigation of the electroosmotic properties indicated a template directed and enthalpy controlled self-assembly of the polyrotaxanes during the polymerisation of the continuous beds. This process was monomer-composition dependent and favored by the hydrophobicity of the polymeric skeleton. The morphology of the continuous beds was evaluated using high-resolution optical microscopy with CCD camera and atomic force microscopy. Reversed-phase capillary chromatography driven by electroosmosis, originating from the polyrotaxane structure, was performed using several test mixtures. Not primarily designed for the chiral chromatography the polyrotaxane derivatized continuous beds demonstrated enantioselective separation of D,L-metoprolol. The stability of the polyrotaxane derivatized continuous beds was tested. The beds demonstrated reproducible electroosmotic properties in the range from pH 4 to pH 9 (RSD=0.69%). (C) 2002 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:225 / 235
页数:11
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