Surface plasmon resonance immunosensor for the detection of Salmonella typhimurium

被引:176
作者
Oh, BK
Kim, YK
Park, KW
Lee, WH
Choi, JW
机构
[1] Sogang Univ, Dept Chem & Biomol Engn, Seoul 121742, South Korea
[2] Hankyong Natl Univ, Dept Chem Engn, Ansung 456749, Kyonggi Do, South Korea
关键词
immunosensor; surface plasmon resonance; protein G; self-assembly monolayer; Salmonella typhimurium;
D O I
10.1016/j.bios.2003.12.009
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
An immunosensor based on surface plasmon resonance (SPR) using protein G was developed for the detection of Salmonella typhimurium. A protein G layer was fabricated by binding chemically to self-assembly monolayer (SAM) of 11-mercaptoundecanoic acid (MUA) on gold (Au) surface. The formation of protein G layer on Au surface modified with 11-MUA and the binding of antibody and antigen in series were confirmed by SPR spectroscopy. The effect of detergent such as Tween-20 on binding efficiency of antibody and antigen was investigated by SPR. The binding efficiency of antigen to the antibody immobilized on Au surface was improved up to about 85% and 100% by using protein G and Tween-20, respectively. The surface morphology analyses of 11-MUA monolayer on An substrate, protein G layer on 11-MUA monolayer and antibody layer immobilized on protein G layer were performed by atomic force microscope (AFM). Consequently, an immunosensor based on SPR for the detection of S. typhimurium using protein G was developed with a detection range of 10(2) to 10(9) CFU/ml. The current fabrication technique of a SPR immunosensor for the detection of S. typhimurium could be applied to construct other immnosensors or protein chips. (C) 2003 Elsevier B.V. All rights reserved.
引用
收藏
页码:1497 / 1504
页数:8
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