Simultaneous analysis of tocopherols, cholesterol, and phytosterols using gas chromatography

被引：93

作者：

Du, M ^{[1
]}

Ahn, DU ^{[1
]}

机构：

[1] Iowa State Univ, Dept Anim Sci, Ames, IA 50011 USA

来源：

JOURNAL OF FOOD SCIENCE | 2002年 / 67卷 / 05期

关键词：

tocopherols; cholesterol; phytosterols; gas chromatography; food products;

D O I：

10.1111/j.1365-2621.2002.tb08708.x

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

A method for the simultaneous analysis of tocopherols and sterols was developed. Lipids were extracted with the Folch method, saponified by KOH-ethanol, and then tocopherols, cholesterol, and phytosterols were extracted with hexane. The extracted samples were dried under a nitrogen stream, derivatized using trimethylsilyl compounds, and then subjected to a gas chromatography. The recovery rates for cholesterol, stigmasterol, and sitosterol were about 100%, but recovery rates for tocopherols were low (25% for delta-tocopherol and 66% for gamma-tocopherol) and varied according to compound structures. However, the recovery rates for delta- and gamma-tocopherols increased to about 100% when the amounts of water and hexane were increased to 15 mL at the extraction step after saponification.

引用

页码：1696 / 1700

页数：5

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