Human autoantibodies to a novel Golgi protein golgin-67: High similarity with golgin-95/gm 130 autoantigen

被引:25
作者
Eystathioy, T
Jakymiw, A
Fujita, DJ
Fritzler, MJ
Chan, EKL
机构
[1] Scripps Res Inst, Dept Mol & Expt Med, Wm Keck Autoimmune Dis Ctr, La Jolla, CA 92037 USA
[2] Univ Calgary, Med Ctr, Dept Biochem & Mol Biol, Canc Biol Res Grp, Calgary, AB, Canada
[3] Univ Calgary, Dept Med, Calgary, AB, Canada
[4] Univ Calgary, Dept Med Biochem, Calgary, AB, Canada
关键词
autoantibody; autoantigen; autoimmunity; golgi complex;
D O I
10.1006/jaut.1999.0359
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Autoantibodies to subcellular organelles have been described in patients with various systemic rheumatic diseases and our laboratories have been focused on studies of the Golgi complex as the autoimmune target. We have previously isolated and described four of the five known Golgi autoantigens reported to date. During the characterization of Golgi autoantigen golgin-95/gm130, another human cDNA that shared a significant degree of similarity in both nucleotide and amino acid sequences was identified. Analysis of cDNAs from different libraries suggested that this is a distinct gene encoding a protein of 67 kDa which has four regions with sequence identity to gm130; ranging between 42 and 60%. Ln this report, we describe the complete cDNA encoding a closely related Golgi protein provisionally named golgin-67. Among a group of 84 human anti-Golgi sera, five (6%) were shown to recognize golgin-67. Anti-golgin-67 human sera and affinity purified rabbit antibody to the recombinant protein gave predominant Golgi staining. Golgin-67 is thus the smallest member of a growing family of Golgi autoantigens rich in alpha-helical coiled-coil domain. The current hypothesis for the generation of autoimmune antibody to the Golgi complex is discussed. (C) 2000 Academic Press.
引用
收藏
页码:179 / 187
页数:9
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