Quantitative trait locus mapping of airway responsiveness to chromosomes 6 and 7 in inbred mice

被引:49
作者
De Sanctis, GT
Singer, JB
Jiao, A
Yandava, CN
Lee, YH
Haynes, TC
Lander, ES
Beier, DR
Drazen, JM
机构
[1] Brigham & Womens Hosp, Div Pulm Med, Combined Program Pulm & Crit Care Med, Boston, MA 02115 USA
[2] Brigham & Womens Hosp, Div Genet, Boston, MA 02115 USA
[3] Harvard Univ, Sch Med, Boston, MA 02115 USA
[4] MIT, Whitehead Inst Biomed Res, Ctr Genome Res, Cambridge, MA 02142 USA
[5] MIT, Dept Biol, Cambridge, MA 02139 USA
[6] Jackson Lab, Bar Harbor, ME 04609 USA
关键词
asthma; methacholine; A/J mice; C3H/HeJ mice;
D O I
10.1152/ajplung.1999.277.6.L1118
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
Quantitative trait locus (QTL) mapping was used to identify chromosomal regions contributing to airway hyperresponsiveness in mice. Airway responsiveness to methacholine was measured in A/J and C3H/HeJ parental strains as well as in progeny derived from crosses between these strains. QTL mapping of backcross [(A/J x C3H/HeJ) x C3H/HeJ] progeny (n = 137-227 informative mice for markers tested) revealed two significant Linkages to loci on chromosomes 6 and 7. The QTL on chromosome 6 confirms the previous report by others of a linkage in this region in the same genetic backgrounds; the second QTL, on chromosome 7, represents a novel locus. In addition, we obtained suggestive evidence for linkage (logarithm of odds ratio = 1.7) on chromosome 17, which lies in the same region previously identified in a cross between A/J and C57BL/6J mice. Airway responsiveness in a cross between A/J and C3H/HeJ mice is under the control of at least two major genetic loci, with evidence for a third locus that has been previously implicated in an A/J and C57BL/6J cross; this indicates that multiple genetic factors central the expression of this phenotype.
引用
收藏
页码:L1118 / L1123
页数:6
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