Determination of 1-deoxynojirimycin in mulberry leaves using hydrophilic interaction chromatography with evaporative light scattering detection

被引：100

作者：

Kimura, T ^{[1
]}

Nakagawa, K

Saito, Y

Yamagishi, K

Suzuki, M

Yamaki, K

Shinmoto, H

Miyazawa, T

机构：

[1] Natl Agr Res Ctr, Fukushima 9602156, Japan

[2] Tohoku Univ, Grad Sch Life Sci & Agr, Food & Biodynam Chem Lab, Sendai, Miyagi 9818555, Japan

[3] Fukushima Technol Ctr, Aizuwakamatsu Tech Support Ctr, Aizu Wakamatsu 9650006, Japan

[4] Natl Food Res Inst, Tsukuba, Ibaraki 3058642, Japan

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2004年 / 52卷 / 06期

关键词：

1-deoxynojirimycin; evaporative light scattering detector; mulberry leaves; Morus alba;

D O I：

10.1021/jf0306901

中图分类号：

S [农业科学];

学科分类号：

09 [农学];

摘要：

A simple and rapid method for determining 1-deoxynojirimycin (DNJ), a potent glucosidase inihibitor present in mulberry leaves (Morus alba and Morus bombysis), by high-performance liquid chromatography coupled to an evaporative light scattering detector (ELSD) has been developed. DNJ was separated from an extract of mulberry leaves on a TSKgel Amide-80 column, which is a representative column for hydrophilic interaction chromatography. During postcolumn detection, DNJ was detected by ELSD and concurrently identified by mass spectrometry. The detection limit was 100 ng. This method is sufficiently sensitive for determining DNJ in mulberry leaves and other related products.

引用

页码：1415 / 1418

页数：4

共 25 条

[1]

HYDROPHILIC-INTERACTION CHROMATOGRAPHY OF COMPLEX CARBOHYDRATES [J].