Real Time Polymerase Chain Reaction (PCR) quantification of T25 maize seeds - influence of the genetic structures in the maize kernel on the quantitative analysis

被引：8

作者：

Papazova, N.

Taverniers, I.

Degrieck, I.

Van Bockstaele, E.

Joost, H.

De Loose, M.

机构：

[1] Inst Agr & Fisheries Res, Unit Technol & Food, B-9820 Merelbeke, Belgium

[2] AgroBioInst, Sofia 1164, Bulgaria

[3] Inst Agr & Fisheries Res, Unit Plant, B-9090 Melle, Belgium

[4] Univ Ghent, Fac Agr & Appl Biol Sci, Dept Plant Prod, B-9000 Ghent, Belgium

[5] Bayer BioSci, B-9052 Ghent, Belgium

来源：

SEED SCIENCE AND TECHNOLOGY | 2006年 / 34卷 / 02期

关键词：

D O I：

10.15258/sst.2006.34.2.06

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Real Time PCR is a technique widely used for GMO quantification. The maize seed contains diploid embryo and triploid endosperm. This implies that the ratio transgene/endogene determined by Real Time PCR will vary due to the presence of different genome copies in the seed compartments. The transgene/endogene ratio is determined for single seeds from commercial transgenic line T25. This ratio is compared between the seeds that originate from reciprocal crosses between T25 and the conventional variety B73. The variation in the GMO content due to the presence of seeds with different origin in the seed samples of 3000 seeds is evaluated.

引用

页码：307 / 317

页数：11

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