Cloning and functional expression of a cDNA encoding a mouse type 2 neuropeptide Y receptor

被引：35

作者：

Nakamura, M

Aoki, Y

Hirano, D

机构：

[1] Life Science Research Laboratory, Japan Tobacco Inc., Yokohama, Kanagawa 227, 6-2 Umegaoka, Aoba-ku

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES | 1996年 / 1284卷 / 02期

关键词：

molecular cloning; neuropeptide Y; Y2-type receptor; (mouse);

D O I：

10.1016/S0005-2736(96)00166-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A cDNA clone homologous with the human neuropeptide Y (NPY)-Y2 receptor has been isolated from a mouse brain cDNA library. Analysis of the predicted amino-acid sequence indicates that the polypeptide encoded by this cDNA is 94% homologous to the human NPY-Y2 receptor. In Chinese hamster ovary (CHO) cells expressing the mouse NPY-Y2 receptor, an increase in intracellular Ca2+ and inhibition of forskolin-induced cAMP accumulation were observed due to stimulation with NPY. NPY-(13-36) and peptide YY, but not with pancreatic polypeptide or [Leu(31), Pro(34)]NPY. The fact that the NPY-induced increase in intracellular Ca2+ and inhibition of forskolin-induced cAMP accumulation were eliminated by pretreatment with pertussis toxin suggests that the NPY-Y2 receptor couples to PTX-sensitive G-proteins(s), probably Gi/Go, in CHO cells.

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页码：134 / 137

页数：4

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