Plaque assay for African swine fever virus on swine macrophages - Brief report

被引:10
作者
Bustos, MJ [1 ]
Nogal, ML [1 ]
Revilla, Y [1 ]
Carrascosa, AL [1 ]
机构
[1] Univ Autonoma Madrid, Ctr Biol Mol Severo Ochoa, CSIC, UAM, Madrid, Spain
关键词
D O I
10.1007/s00705-002-0814-6
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A plaque assay developed to detect the infection of African Swine Fever Virus on swine macrophages is described. Plaques were generated by all of the virus isolates tested. The method is suitable not only for virus titration but also for the selection of:clones in protocols for isolation/purification of recombinant viruses.
引用
收藏
页码:1453 / 1459
页数:7
相关论文
共 15 条
[1]   INTERACTION OF AFRICAN SWINE FEVER VIRUS WITH MACROPHAGES [J].
ALCAMI, A ;
CARRASCOSA, AL ;
VINUELA, E .
VIRUS RESEARCH, 1990, 17 (02) :93-104
[2]   PRODUCTION AND TITRATION OF AFRICAN SWINE FEVER VIRUS IN PORCINE ALVEOLAR MACROPHAGES [J].
CARRASCOSA, AL ;
SANTAREN, JF ;
VINUELA, E .
JOURNAL OF VIROLOGICAL METHODS, 1982, 3 (06) :303-310
[3]   Virus-specific cell receptors are necessary, but not sufficient, to confer cell susceptibility to African swine fever virus [J].
Carrascosa, AL ;
Bustos, MJ ;
Galindo, I ;
Viñuela, E .
ARCHIVES OF VIROLOGY, 1999, 144 (07) :1309-1321
[4]   TITRATION OF AFRICAN SWINE FEVER (ASF) VIRUS [J].
ENJUANES, L ;
CARRASCOSA, AL ;
MORENO, MA ;
VINUELA, E .
JOURNAL OF GENERAL VIROLOGY, 1976, 32 (SEP) :471-477
[5]   African swine fever virus EP153R open reading frame encodes a glycoprotein involved in the hemadsorption of infected cells [J].
Galindo, I ;
Almazán, F ;
Bustos, MJ ;
Viñuela, E ;
Carrascosa, AL .
VIROLOGY, 2000, 266 (02) :340-351
[6]   IMPROVEMENT OF AFRICAN SWINE-FEVER VIRUS NEUTRALIZATION ASSAY USING RECOMBINANT VIRUSES EXPRESSING CHROMOGENIC MARKER GENES [J].
GOMEZPUERTAS, P ;
RODRIGUEZ, F ;
ORTEGA, A ;
OVIEDO, JM ;
ALONSO, C ;
ESCRIBANO, JM .
JOURNAL OF VIROLOGICAL METHODS, 1995, 55 (02) :271-279
[7]  
Hess W. R., 1960, Bulletin of epizootic diseases of Africa, V8, P317
[8]  
MALMQUIST WA, 1960, AM J VET RES, V21, P104
[9]   A BIR motif containing gene of African swine fever virus, 4CL, is nonessential for growth in vitro and viral virulence [J].
Neilan, JG ;
Lu, Z ;
Kutish, GF ;
Zsak, L ;
Burrage, TG ;
Borca, MV ;
Carrillo, C ;
Rock, DL .
VIROLOGY, 1997, 230 (02) :252-264
[10]   African swine fever virus dUTPase is a highly specific enzyme required for efficient replication in swine macrophages [J].
Oliveros, M ;
García-Escudero, R ;
Alejo, A ;
Viñuela, E ;
Salas, ML ;
Salas, J .
JOURNAL OF VIROLOGY, 1999, 73 (11) :8934-8943