Cell separation by dielectrophoretic field-flow-fractionation

被引:344
作者
Wang, XB [1 ]
Yang, J [1 ]
Huang, Y [1 ]
Vykoukal, J [1 ]
Becker, FF [1 ]
Gascoyne, PRC [1 ]
机构
[1] Univ Texas, MD Anderson Canc Ctr, Dept Mol Pathol, Houston, TX 77030 USA
关键词
D O I
10.1021/ac990922o
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Dielectrophoretic field-now-fractionation (DEP-FFF) was applied to several clinically relevant cell separation problems, including the purging of human breast cancer cells from normal T-lymphocytes and from CD34(+) hematopoietic stem cells, the separation of the major leukocyte subpopulations, and the enrichment of leukocytes from blood. Cell separations were achieved in a thin chamber equipped with a microfabricated, interdigitated electrode array on its bottom wall that was energized with AC electric signals. Cells were levitated by the balance between DEP and sedimentation forces to different equilibrium heights and were transported at differing velocities and thereby separated when a velocity profile was established in the chamber. This bulk-separation technique adds cell intrinsic dielectric properties to the catalog of physical characteristics that can be applied to cell discrimination. The separation process and performance can be controlled through electronic means. Cell labeling is unnecessary, and separated cells may be cultured and further analyzed. It can be scaled up for routine laboratory cell separation or implemented on a miniaturized scale.
引用
收藏
页码:832 / 839
页数:8
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