Aqueous two-phase extraction for downstream processing of amyloglucosidase

被引：46

作者：

Tanuja, S

Srinivas, ND

Rao, KSMSR

Gowthaman, MK

机构：

[1] CENT FOOD TECHNOL RES INST, DEPT FOOD ENGN, MYSORE 570013, KARNATAKA, INDIA

[2] CENT FOOD TECHNOL RES INST, DEPT FERMENTAT TECHNOL, MYSORE 570013, KARNATAKA, INDIA

来源：

PROCESS BIOCHEMISTRY | 1997年 / 32卷 / 08期

关键词：

D O I：

10.1016/S0032-9592(97)00009-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A polymer/salt aqueous two-phase system has been successfully employed for separation and purification of amyloglucosidase. The effects of system pH, molecular weight of polymer and composition of the two-phase system on amyloglucosidase partition behaviour in polyethylene glycol (PEG 4000, 6000)/disodium hydrogen phosphate were investigated. Experimental data are explained based on Kim's theoretical model for the prediction of biomolecule partitioning in a PEG/salt system. (C) 1997 Elsevier Science Ltd.

引用

页码：635 / 641

页数：7

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