Fluorescence-based nitric oxide detection by ruthenium porphyrin fluorophore complexes

被引：83

作者：

Lim, MH ^{[1
]}

Lippard, SJ ^{[1
]}

机构：

[1] MIT, Dept Chem, Cambridge, MA 02139 USA

来源：

INORGANIC CHEMISTRY | 2004年 / 43卷 / 20期

关键词：

D O I：

10.1021/ic035418n

中图分类号：

O61 [无机化学];

学科分类号：

070301 ; 081704 ;

摘要：

The ruthenium(II) porphyrin fluorophore complexes [Ru(TPP)(CO)(Ds-R)] (TPP = tetraphenylporphinato dianion; Ds = dansyl; R = imidazole (im), 1, or thiomorpholine (tm), 2) were synthesized and investigated for their ability to detect nitric oxide (NO) based on fluorescence. The X-ray crystal structures of 1 and 2 were determined. The Ds-im or Ds-tm ligand coordinates to an axial site of the ruthenium(II) center through a nitrogen or sulfur atom, respectively. Both exhibit quenched fluorescence when excited at 368 or 345 nm. Displacement of the metal-coordinated fluorophore by NO restores fluorescence within minutes. These observations demonstrate fluorescence-based NO detection using ruthenium porphyrin fluorophore conjugates.

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页码：6366 / 6370

页数：5

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