Epidural anaesthesia restores pancreatic microcirculation and decreases the severity of acute pancreatitis

AIM: To investigate the effect of epidural anaesthesia (EA) on pancreatic microcirculation during acute pancreatitis (AP). METHODS: AP was induced by injection of sodium taurocholate into the pancreatic duct of Sprague-Dawley rats. To realize EA, a catheter was introduced into the epidural space between T7 and T9 and bupivacaine was injected. Microcirculatory flow was measured by laser Doppler flowmetry. Arterial blood gas analyses were performed. At the end of the experiment (<= 5 h), pancreas was removed for histology. The animals were divided into three groups: Group 1 (n = 9), AP without EA; Group 2 (n = 4), EA without AP; and Group 3 (n = 6), AP treated by EA. RESULTS: In Group 1, pancreatic microcirculatory flow prior to AP was 141 +/- 39 perfusion units (PU). After AP, microcirculatory flow obviously decreased to 9 +/- 6 PU (P < 0.05). Metabolic acidosis developed with base excess (BE) of - 14 +/- 3 mmol/L. Histology revealed extensive edema and tissue necrosis. In Group 2, EA did not significantly modify microcirculatory flow. BE remained unchanged and histological analysis showed normal pancreatic tissue. In Group 3, AP initially caused a significant decrease in microcirculatory flow from 155 +/- 25 to 11 +/- 7 PU (P < 0.05). After initiation of EA, microcirculatory