An integral protein of the inner nuclear membrane localizes to the mitotic spindle in mammalian cells

被引:61
作者
Buch, Charlotta [1 ,2 ]
Lindberg, Robert [1 ,3 ]
Figueroa, Ricardo [1 ,3 ]
Gudise, Santhosh [1 ,2 ]
Onischenko, Evgeny [4 ]
Hallberg, Einar [1 ]
机构
[1] Sodertorns Univ, SE-14189 Huddinge, Sweden
[2] Karolinska Inst, Dept Biosci & Nutr, SE-14157 Huddinge, Sweden
[3] Stockholm Univ, Dept Biochem & Biophys, SE-10691 Stockholm, Sweden
[4] Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA
基金
瑞典研究理事会;
关键词
Inner nuclear membrane; Transmembrane proteins; Mitotic spindle; Mitosis; Cancer; IN-VIVO; ENVELOPE; MATRIX; MITOSIS; INTERACTS; SKELETOR; MEGATOR; COMPLEX; REDISTRIBUTES; MICROTUBULES;
D O I
10.1242/jcs.047373
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Here, we characterize a transmembrane protein of the nuclear envelope that we name spindle-associated membrane protein 1 (Samp1). The protein is conserved in metazoa and fission yeast and is homologous to Net5 in rat and Ima1 in Schizosaccharomyces pombe. We show that, in human cells, the protein is a membrane-spanning polypeptide with an apparent molecular mass of 43 kDa. This is consistent with a predicted polypeptide of 392 amino acids that has five transmembrane segments and its C-terminus exposed to the nucleoplasm. During interphase, Samp1 was specifically distributed in the inner nuclear membrane. Post-transcriptional silencing of Samp1 expression resulted in separation of centrosomes from the nuclear envelope, indicating that it is functionally connected to the cytoskeleton. At the onset of mitosis, most of the protein dispersed out into the ER, as expected. However, during mitosis, a significant fraction of the protein specifically localized to the polar regions of the mitotic spindle. We demonstrate for the first time, in human cells, the existence of a membranous structure overlapping with the mitotic spindle. Interestingly, another integral inner nuclear membrane protein, emerin, was absent from the spindle-associated membranes. Thus, Samp1 defines a specific membrane domain associated with the mitotic spindle.
引用
收藏
页码:2100 / 2107
页数:8
相关论文
共 28 条
[1]   The life cycle of the metazoan nuclear envelope [J].
Anderson, Daniel J. ;
Hetzer, Martin W. .
CURRENT OPINION IN CELL BIOLOGY, 2008, 20 (04) :386-392
[2]   An evolutionarily conserved NPC subcomplex, which redistributes in part to kinetochores in mammalian cells [J].
Belgareh, N ;
Rabut, G ;
Baï, SW ;
van Overbeek, M ;
Beaudouin, J ;
Daigle, N ;
Zatsepina, OV ;
Pasteau, F ;
Labas, V ;
Fromont-Racine, M ;
Ellenberg, J ;
Doye, V .
JOURNAL OF CELL BIOLOGY, 2001, 154 (06) :1147-1160
[3]   The nuclear envelope as an integrator of nuclear and cytoplasmic architecture [J].
Crisp, Melissa ;
Burke, Brian .
FEBS LETTERS, 2008, 582 (14) :2023-2032
[4]   Distribution of emerin during the cell cycle [J].
Dabauvalle, MC ;
Müller, E ;
Ewald, A ;
Kress, W ;
Krohne, G ;
Müller, CR .
EUROPEAN JOURNAL OF CELL BIOLOGY, 1999, 78 (10) :749-756
[5]   Nuclear membrane dynamics and reassembly in living cells: Targeting of an inner nuclear membrane protein in interphase and mitosis [J].
Ellenberg, J ;
Siggia, ED ;
Moreira, JE ;
Smith, CL ;
Presley, JF ;
Worman, HJ ;
LippincottSchwartz, J .
JOURNAL OF CELL BIOLOGY, 1997, 138 (06) :1193-1206
[6]   Titin in insect spermatocyte spindle fibers associates with microtubules, actin, myosin and the matrix proteins skeletor, megator and chromator [J].
Fabian, Lacramioara ;
Xia, Xuequin ;
Venkitaramani, Deepa V. ;
Johansen, Kristen M. ;
Johansen, Jorgen ;
Andrew, Deborah J. ;
Forer, Arthur .
JOURNAL OF CELL SCIENCE, 2007, 120 (13) :2190-2204
[7]   IDENTIFICATION OF A MAJOR POLYPEPTIDE OF THE NUCLEAR-PORE COMPLEX [J].
GERACE, L ;
OTTAVIANO, Y ;
KONDORKOCH, C .
JOURNAL OF CELL BIOLOGY, 1982, 95 (03) :826-837
[8]   NUCLEAR-ENVELOPE LAMINA IS REVERSIBLY DEPOLYMERIZED DURING MITOSIS [J].
GERACE, L ;
BLOBEL, G .
CELL, 1980, 19 (01) :277-287
[9]   Importin beta: Conducting a much larger cellular symphony [J].
Harel, A ;
Forbes, DJ .
MOLECULAR CELL, 2004, 16 (03) :319-330
[10]   Cell and molecular biology of the spindle matrix [J].
Johansen, Kristen M. ;
Johansen, Jorgen .
INTERNATIONAL REVIEW OF CYTOLOGY - A SURVEY OF CELL BIOLOGY, VOL 263, 2007, 263 :155-+