Sequence and functional analysis of the gene encoding Vibrio cholerae cAMP receptor protein

被引：13

作者：

Skorupski, K

Taylor, RK

机构：

来源：

GENE | 1997年 / 198卷 / 1-2期

关键词：

CRP; cholera toxin; virulence; chromosomal capture;

D O I：

10.1016/S0378-1119(97)00331-4

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We describe here the cloning, nucleotide sequence, and functional expression of the clp gene of Vibrio cholerae (Vc) encoding the cyclic AMP receptor protein (CRP). The Ve crp gene shows 81% identity with the crp gene from Escherichia coli (Ec) and its deduced amino acid sequence shows 95% identity with the Ec protein. When expressed from inducible promoters, the cloned Ve gene produced an approximately 20-kDa protein which complemented the carbohydrate-negative and growth-defective phenotypes of both Ec and Ve crp mutants. In the Vc clp mutant, the cloned crp gene also restored the normal repression of ToxR-regulated virulence genes which occurs under certain environmental conditions. (C) 1997 Elsevier Science B.V.

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收藏

页码：297 / 303

页数：7

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