Differential NF-κB pathways induction by Lactobacillus plantarum in the duodenum of healthy humans correlating with immune tolerance

被引：308

作者：

van Baarlen, Peter ^{[1
,2
]}

Troost, Freddy J. ^{[1
,3
]}

van Hemert, Saskia ^{[1
,4
]}

van der Meer, Cindy ^{[1
,4
]}

de Vos, Willem M. ^{[5
,6
]}

de Groot, Philip J. ^{[1
,7
]}

Hooiveld, Guido J. E. J. ^{[1
,7
]}

Brummer, Robert-Jan M. ^{[1
,3
]}

Kleerebezem, Michiel ^{[1
,4
,5
]}

机构：

[1] Top Inst Food & Nutr, NL-6700 AN Wageningen, Netherlands

[2] Radboud Univ Nijmegen, Med Ctr, Ctr Mol & Biomol Informat, NL-6500 HB Nijmegen, Netherlands

[3] Maastricht Univ, Dept Internal Med, Div Gastroenterol, NL-6200 MD Maastricht, Netherlands

[4] NIZO Food Res, NL-6710 BA Ede, Netherlands

[5] Wageningen Univ, Lab Microbiol Agrotechnol & Food Sci, NL-6703 HB Wageningen, Netherlands

[6] Univ Helsinki, Dept Vet Basic Sci, FI-00014 Helsinki, Finland

[7] Wageningen Univ, Div Human Nutr, Nutr Metab & Genom Grp, NL-6700 EV Wageningen, Netherlands

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2009年 / 106卷 / 07期

关键词：

commensal bacteria; expression profiling; host-microbe interactions; pathway analysis; ADAPTIVE IMMUNITY; DENDRITIC CELLS; TEICHOIC-ACIDS; EXPRESSION; BACTERIA; RECEPTOR; STREPTOCOCCI; INFLAMMATION; RECOGNITION; MONOCYTES;

D O I：

10.1073/pnas.0809919106

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

How do we acquire immune tolerance against food microorganisms and commensal bacteria that constitute the intestinal microbiota? We investigated this by stimulating the immune system of adults with commensal Lactobacillus plantarum bacteria. We studied the in vivo human responses to L. plantarum in a randomized double-blind placebo-controlled cross-over study. Healthy adults ingested preparations of living and heat-killed L. plantarum bacteria. Biopsies were taken from the intestinal duodenal mucosa and altered expression profiles were analyzed using whole-genome microarrays and by biological pathway reconstructions. Expression profiles of human mucosa displayed striking differences in modulation of NF-kappa B-dependent pathways, notably after consumption of living L. plantarum bacteria in different growth phases. Our in vivo study identified mucosal gene expression patterns and cellular pathways that correlated with the establishment of immune tolerance in healthy adults.

引用

页码：2371 / 2376

页数：6

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