Amplified Fluorescence Turn-On Assay for Mercury(II) Detection and Quantification based on Conjugated Polymer and Silica Nanoparticles

被引：35

作者：

Wang, Yusong ^{[1
]}

Liu, Bin ^{[1
]}

机构：

[1] Natl Univ Singapore, Dept Chem & Biomol Engn, Singapore 117576, Singapore

来源：

MACROMOLECULAR RAPID COMMUNICATIONS | 2009年 / 30卷 / 07期

关键词：

conjugated polymers; fluorescence; mercury (II); nanoparticles; sensors; silicas; SELECTIVE DETECTION; AQUEOUS-SOLUTIONS; DNA DETECTION; COLORIMETRIC DETECTION; SIGNAL AMPLIFICATION; GOLD NANOPARTICLES; OPTICAL-DETECTION; MERCURIC ION; HG-II; SENSOR;

D O I：

10.1002/marc.200800727

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

An amplified fluorescence turn-on assay for mercury(II) detection and quantification was developed. This method makes use of specific thymine/mercury(II)/thymine coordination to capture Fl-labeled DNA onto NP surface. Addition of a cationic conjugated polymer leads to an amplified Fl signal in solution. A sigmoidal Hg2+ working curve is obtained at fixed [NP] with a detection limit of 0.1 x 10(-6) M. However, by reducing [Hg2+] and [NP] simultaneously, while maintaining [Hg2+]:[DNA duplex] = 3:1, a linear calibration curve is observed with a detection limit of 5 x 10(-9) M. The CCP-assisted mercury(II) assay shows potential applications in environmental mercury detection and for industrial process control.

引用

页码：498 / 503

页数：6

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