Sequence preference of 7,12-dimethylbenz[a]anthracene-syn-diol epoxide-DNA binding in the mouse H-ras gene detected by UvrABC nucleases

被引:15
作者
Chen, JX
Pao, A
Zheng, Y
Ye, XC
Kisleyou, AS
Morris, R
Slaga, TJ
Harvey, RG
Tang, MS
机构
[1] UNIV TEXAS,MD ANDERSON CANC CTR,DEPT CARCINOGENESIS,DIV SCI PK RES,SMITHVILLE,TX 78975
[2] LANKENAU MED RES CTR,WYNNEWOOD,PA 19096
[3] UNIV CHICAGO,BEN MAY INST,CHICAGO,IL 60637
关键词
D O I
10.1021/bi9604136
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have found that 7,12-dimethylbenz[a]anthracene-syn-diol epoxide (syn-DMBADE)-modified DNA fragments are sensitive to UvrABC incision. The incisions occur mainly seven bases 5' and four bases 3' of a syn-DMBADE-modified adenine or guanine residue. The kinetics of UVrABC incision at different sequences in a DNA fragment are the same, and the extent of UVrABC incision is proportional to the syn-DMBADE concentration. On the basis of these results, we have concluded that UvrABC incision on syn-DMBADE-DNA adducts is independent of DNA sequence and is quantitative. Using the UvrABC incision method, we have analyzed the syn-DMBADE-DNA binding spectrum in several defined DNA fragments, including the first two exons of the mouse H-ras gene. We have found that both guanine and adenine residues in codons 12, 13, and 61 of the H-ras gene are strong syn-DMBADE binding sites. These results suggest that the initial binding of DMBADE may greatly contribute to the frequency of H-ras mutations. Results from dinucleotide binding analysis indicate that the 5'-nearest neighbor displays a greater effect on syn-DMBADE-DNA binding than the 3'-nearest neighbor.
引用
收藏
页码:9594 / 9602
页数:9
相关论文
共 45 条
[1]   ONCOGENE ACTIVATION IN CHEMICAL CARCINOGENESIS [J].
BALMAIN, A ;
BROWN, K .
ADVANCES IN CANCER RESEARCH, 1988, 51 :147-182
[2]   RAS GENES [J].
BARBACID, M .
ANNUAL REVIEW OF BIOCHEMISTRY, 1987, 56 :779-827
[3]   VARIATION IN ROUTE OF MICROSOMAL ACTIVATION OF 7,12-DIMETHYLBENZ[A]ANTHRACENE WITH SUBSTRATE CONCENTRATION [J].
BIGGER, CAH ;
TOMASZEWSKI, JE ;
DIPPLE, A .
CARCINOGENESIS, 1980, 1 (01) :15-20
[4]   LIMITATIONS OF METABOLIC-ACTIVATION SYSTEMS USED WITH INVITRO TESTS FOR CARCINOGENS [J].
BIGGER, CAH ;
TOMASZEWSKI, JE ;
DIPPLE, A ;
LAKE, RS .
SCIENCE, 1980, 209 (4455) :503-505
[5]  
BIGGER CAH, 1983, CANCER RES, V43, P5647
[6]   MUTAGENESIS OF THE HA-RAS ONCOGENE IN MOUSE SKIN TUMORS INDUCED BY POLYCYCLIC AROMATIC-HYDROCARBONS [J].
BIZUB, D ;
WOOD, AW ;
SKALKA, AM .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (16) :6048-6052
[7]  
BOS JL, 1989, CANCER RES, V49, P4682
[8]   ISOLATION AND CHARACTERIZATION OF THE 5' FLANKING REGION OF THE MOUSE C-HARVEY-RAS GENE [J].
BROWN, K ;
BAILLEUL, B ;
RAMSDEN, M ;
FEE, F ;
KRUMLAUF, R ;
BALMAIN, A .
MOLECULAR CARCINOGENESIS, 1988, 1 (03) :161-170
[9]   A METABOLITE OF THE CARCINOGEN 7,12-DIMETHYLBENZ[A]ANTHRACENE THAT REACTS PREDOMINANTLY WITH ADENINE RESIDUES IN DNA [J].
CHENG, SC ;
PRAKASH, AS ;
PIGOTT, MA ;
HILTON, BD ;
LEE, H ;
HARVEY, RG ;
DIPPLE, A .
CARCINOGENESIS, 1988, 9 (09) :1721-1723
[10]   ADDITIONAL EVIDENCE FOR THE INVOLVEMENT OF THE 3,4-DIOL 1,2-OXIDES IN THE METABOLIC-ACTIVATION OF 7,12-DIMETHYLBENZ[ALPHA]ANTHRACENE IN MOUSE SKIN [J].
COOPER, CS ;
RIBEIRO, O ;
HEWER, A ;
WALSH, C ;
GROVER, PL ;
SIMS, P .
CHEMICO-BIOLOGICAL INTERACTIONS, 1980, 29 (03) :357-367