Antibody-based fluorescence detection of kinase activity on a peptide array

被引：100

作者：

Lesaicherre, ML

Uttamchandani, M

Chen, GYJ

Yao, SQ

机构：

[1] Natl Univ Singapore, Dept Chem, Singapore 117543, Singapore

[2] Natl Univ Singapore, Dept Biol Sci, Singapore 117543, Singapore

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2002年 / 12卷 / 16期

关键词：

D O I：

10.1016/S0960-894X(02)00378-5

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Peptide-based microarrays allow for high-throughput identification of protein kinase substrates. However, current methods of detecting kinase activity require the use of radioisotopes. We have developed a novel fluorescence-based approach for quantitative detection of peptide phosphorylation on chip using fluorescently-labeled anti-phosphoserine and anti-phosphotyrosine antibodies. This method is sensitive, specific and extremely fast, presenting obvious advantages and may find wider uses in high-throughput kinase screenings. (C) 2002 Elsevier Science Ltd. All rights reserved.

引用

页码：2085 / 2088

页数：4

共 16 条

[1] Toward the phosphoproteome
Ahn, NG
Resing, KA
[J]. NATURE BIOTECHNOLOGY, 2001, 19 (04) : 317 - 318
[2] DDVIR A, 1991, J CELL BIOL, V113, P857
[3] Highly specific, membrane-permeant peptide blockers of cGMP-dependent protein kinase Iα inhibit NO-induced cerebral dilation
Dostmann, WRG
Taylor, MS
Nickl, CK
Brayden, JE
Frank, R
Tegge, WJ
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (26) : 14772 - 14777
[4] Peptide and small molecule microarray for high throughput cell adhesion and functional assays
Falsey, JR
Renil, M
Park, S
Li, SJ
Lam, KS
[J]. BIOCONJUGATE CHEMISTRY, 2001, 12 (03) : 346 - 353
[5] GENERATION AND USE OF SYNTHETIC PEPTIDE COMBINATORIAL LIBRARIES FOR BASIC RESEARCH AND DRUG DISCOVERY
HOUGHTEN, RA
PINILLA, C
BLONDELLE, SE
APPEL, JR
DOOLEY, CT
CUERVO, JH
[J]. NATURE, 1991, 354 (6348) : 84 - 86
[6] Peptide chips for the quantitative evaluation of protein kinase activity
Houseman, BT
Huh, JH
Kron, SJ
Mrksich, M
[J]. NATURE BIOTECHNOLOGY, 2002, 20 (03) : 270 - 274
[7] Kaufmann H, 2001, PROTEOMICS, V1, P194, DOI 10.1002/1615-9861(200102)1:2<194::AID-PROT194>3.3.CO
[8] 2-B
[9] The ''one-bead-one-compound'' combinatorial library method
Lam, KS
Lebl, M
Krchnak, V
[J]. CHEMICAL REVIEWS, 1997, 97 (02) : 411 - 448
[10] Printing small molecules as microarrays and detecting protein-ligand interactions en masse
MacBeath, G
Koehler, AN
Schreiber, SL
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1999, 121 (34) : 7967 - 7968

← 1 2 →