Polymorphic microsatellite loci for paternity analysis in the Madagascar paradise flycatcher (Terpsiphone mutata:Aves)

被引：24

作者：

Adcock, GJ ^{[1
]}

Mulder, RA

机构：

[1] Univ Melbourne, Dept Zool, Melbourne, Vic 3010, Australia

[2] Australian Natl Univ, Sch Bot & Zool, Evolutionary Ecol Grp, Canberra, ACT, Australia

来源：

MOLECULAR ECOLOGY NOTES | 2002年 / 2卷 / 03期

关键词：

Madagascar paradise flycatcher; microsatellite; paternity; Terpsiphone mutata;

D O I：

10.1046/j.1471-8286.2002.00234.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Nine polymorphic microsatellite loci from the Madagascar paradise flycatcher Terpsiphone mutata were isolated using nonradioactive polymerase chain reaction (PCR)-based techniques to screen an enriched genomic library. Seven polymorphic loci showed no evidence of null alleles and exhibited high levels of variation in 18 unrelated individuals (mean diversity = 0.80, mean number of alleles = 13.6). These loci are therefore suitable for analysis of population structure and paternity (exclusion probability for six unlinked loci = 0.9998).

引用

页码：287 / 289

页数：3

共 7 条

[1]

[Anonymous], 2000, ARLEQUIN SOFTWARE PO

[2] M13-tailed primers improve the readability and usability of microsatellite analyses performed with two different allele-sizing methods [J].

Boutin-Ganache, I ;

Raposo, M ;

Raymond, M ;

Deschepper, CF .

BIOTECHNIQUES, 2001, 31 (01) :24-+

[3]

BRUFORD MW, 1992, MOL GENETIC ANAL POP, P227

[4] Isolation of microsatellite loci from a social lizard, Egernia stokesii, using a modified enrichment procedure [J].

Gardner, MG ;

Cooper, SJB ;

Bull, CM ;

Grant, WN .

JOURNAL OF HEREDITY, 1999, 90 (02) :301-304

[5] Comparisons of three probability formulae for parentage exclusion [J].

Jamieson, A ;

Taylor, SS .

ANIMAL GENETICS, 1997, 28 (06) :397-400

[6]

Lunt DH, 1999, MOL ECOL, V8, P891, DOI 10.1046/j.1365-294X.1999.00636.x

[7]

MULDER RA, 2002, IN PRESS J AVIAN BIO

← 1 →