Induction of apoptosis in two human leukemia cell lines as well as differentiation in human promyelocytic cells by cyanidin-3-O-β-glucopyranoside

被引:70
作者
Fimognari, C
Berti, F
Nüsse, B
Cantelli-Forti, G
Hrelia, P
机构
[1] Univ Bologna, Dept Pharmacol, I-40126 Bologna, Italy
[2] GSF, Flow Cytometry Grp, D-85758 Neuherberg, Germany
关键词
chemoprevention; anthocyanins; cytodifferentiation; apoptosis; human T-lymphoblastoid cells; promyelocytic leukemia cells;
D O I
10.1016/j.bcp.2004.02.021
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Little is known about the potentially chemopreventive mechanisms of anthocyanins apart from their antioxidant activity. We investigated the in vitro capacity of the anthocyanin cyanidin-3-O-beta-glucopyranoside (Cy-g) to induce apoptosis in T-lymphoblastoid, as well as apoptosis and differentiation in HL-60 promyelocytic cells. Although Cy-g-induced apoptosis (as well as necrosis) in the two systems, HL-60 cells were much less sensitive than T-lymphoblastoid cells. Moreover, treatment of HL-60 cells with Cy-g caused differentiation into macrophage-like cells and granulocytes. Concerning the mechanism of action, the induction of apoptosis in Jurkat T cells can be explained by a modulation of p53 and bax protein expression. At the molecular level, the induction of apoptosis and cytodifferentiation in HL-60 cells involved different proteins, thus suggesting that the effects of Cy-g on apoptosis and cytodifferentiation induction are two distinct events. These interesting biological properties should encourage further investigation into the chemopreventive and/or chemotherapeutic potential of Cy-g. (C) 2004 Elsevier Inc. All rights reserved.
引用
收藏
页码:2047 / 2056
页数:10
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