The Kw recombinase, an integrase from Kluyveromyces waltii

被引：11

作者：

Ringrose, L ^{[1
]}

Angrand, PO ^{[1
]}

Stewart, AF ^{[1
]}

机构：

[1] EUROPEAN MOL BIOL LAB,D-69117 HEIDELBERG,GERMANY

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1997年 / 248卷 / 03期

关键词：

site-specific recombinase; FLP recombinase; Kluyveromyces waltii; Kw recombinase;

D O I：

10.1111/j.1432-1033.1997.00903.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Site-specific recombinases of the integrase family share limited amino-acid-sequence similarity, but use a common reaction mechanism to recombine distinct DNA target sites. Here we report the characterisation of the Kw site-specific recombinase, encoded on the 2 mu-like plasmid pKWS1 from the yeast Kluyveromyces waltii. Using in vitro-translated Kw recombinase, we show that the protein is able to bind and to recombine its putative DNA target site. Recombination is conservative and the Kw target site has a spacer of seven base pairs. We show that Kw recombinase is able to mediate recombination in a mammalian cell line, thus, it has potential for use as a tool for genomic manipulation in heterologous systems.

引用

页码：903 / 912

页数：10

共 33 条

[1] EVIDENCE FOR A 2ND CONSERVED ARGININE RESIDUE IN THE INTEGRASE FAMILY OF RECOMBINATION PROTEINS [J].