DEP-FFF: Field-flow fractionation using non-uniform electric fields

被引:67
作者
Markx, GH [1 ]
Rousselet, J [1 ]
Pethig, R [1 ]
机构
[1] UNIV MANCHESTER, INST SCI & TECHNOL, DEPT CHEM ENGN, MANCHESTER M60 1QD, LANCS, ENGLAND
基金
英国生物技术与生命科学研究理事会;
关键词
D O I
10.1080/10826079708005597
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Dielectrophoresis (DEP) - the movement of particles in nonuniform electric fields - can be used in combination with Field Flow Fractionation (FFF) to separate particles with differing dielectric properties. An introduction is given to the technique of DEP-FFF and its application to the separation of cells and other particles. The separation of yeast cells using the subtechniques of steric and hyperlayer DEP-FFF is demonstrated. It is shown that the hyperlayer-DEP-FFF techniques have a number of advantages, including an improved separation efficiency and reduced adhesion to chamber walls. The hyperlayer-DEP-FFF separation technique is also independent of particle size and allows the use of higher medium conductivities than for conventional DEP methods.
引用
收藏
页码:2857 / 2872
页数:16
相关论文
共 27 条
  • [1] ARNOLD WM, 1993, BIOCHIM BIOPHYS ACTA, V1157, P32
  • [2] SEPARATION AND SIZE DISTRIBUTION OF RED-BLOOD-CELLS OF DIVERSE SIZE, SHAPE, AND ORIGIN BY FLOW HYPERLAYER FIELD-FLOW FRACTIONATION
    BARMAN, BN
    ASHWOOD, ER
    GIDDINGS, JC
    [J]. ANALYTICAL BIOCHEMISTRY, 1993, 212 (01) : 35 - 42
  • [3] SEPARATION OF HUMAN BREAST-CANCER CELLS FROM BLOOD BY DIFFERENTIAL DIELECTRIC AFFINITY
    BECKER, FF
    WANG, XB
    HUANG, Y
    PETHIG, R
    VYKOUKAL, J
    GASCOYNE, PRC
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (03) : 860 - 864
  • [4] BIGELOW JC, 1991, ACS SYM SER, V464, P146
  • [5] SEPARATION OF LYMPHOCYTE-B AND LYMPHOCYTE-T BY A HYBRID FIELD-FLOW FRACTIONATION ADHESION CHROMATOGRAPHY TECHNIQUE
    BIGELOW, JC
    GIDDINGS, JC
    NABESHIMA, Y
    TSURUTA, T
    KATAOKA, K
    OKANO, T
    YUI, N
    SAKURAI, Y
    [J]. JOURNAL OF IMMUNOLOGICAL METHODS, 1989, 117 (02) : 289 - 293
  • [6] ELECTRICAL FIELD-FLOW FRACTIONATION OF PROTEINS
    CALDWELL, KD
    GIDDINGS, JC
    MYERS, MN
    KESNER, LF
    [J]. SCIENCE, 1972, 176 (4032) : 296 - &
  • [7] FEASIBILITY STUDY OF DIELECTRICAL FIELD-FLOW FRACTIONATION
    DAVIS, JM
    GIDDINGS, JC
    [J]. SEPARATION SCIENCE AND TECHNOLOGY, 1986, 21 (09) : 969 - 989
  • [8] DIELECTROPHORETIC SEPARATION OF MAMMALIAN-CELLS STUDIED BY COMPUTERIZED IMAGE-ANALYSIS
    GASCOYNE, PRC
    HUANG, Y
    PETHIG, R
    VYKOUKAL, J
    BECKER, FF
    [J]. MEASUREMENT SCIENCE AND TECHNOLOGY, 1992, 3 (05) : 439 - 445
  • [9] FIELD-FLOW FRACTIONATION - ANALYSIS OF MACROMOLECULAR, COLLOIDAL, AND PARTICULATE MATERIALS
    GIDDINGS, JC
    [J]. SCIENCE, 1993, 260 (5113) : 1456 - 1465
  • [10] FLOW FIELD-FLOW FRACTIONATION - NEW METHOD FOR SEPARATING, PURIFYING, AND CHARACTERIZING DIFFUSIVITY OF VIRUSES
    GIDDINGS, JC
    YANG, FJ
    MYERS, MN
    [J]. JOURNAL OF VIROLOGY, 1977, 21 (01) : 131 - 138