Histone H3.3 incorporation provides a unique and functionally essential telomeric chromatin in embryonic stem cells

被引：135

作者：

Wong, Lee H. ^{[1
]}

Ren, Hua ^{[1
]}

Williams, Evan ^{[1
]}

McGhie, James ^{[1
]}

Ahn, Soyeon ^{[1
]}

Sim, Marcus ^{[1
]}

Tam, Angela ^{[1
]}

Earle, Elizabeth ^{[1
]}

Anderson, Melissa A. ^{[1
]}

Mann, Jeffrey ^{[2
]}

Choo, K. H. Andy ^{[1
]}

机构：

[1] Univ Melbourne, Chromosome & Chromatin Res Lab, Murdoch Childrens Res Inst, Dept Paediat,Royal Childrens Hosp, Parkville, Vic 3052, Australia

[2] Univ Melbourne, Stem Cell Epigenet Res Lab, Murdoch Childrens Res Inst, Dept Paediat,Royal Childrens Hosp, Parkville, Vic 3052, Australia

来源：

GENOME RESEARCH | 2009年 / 19卷 / 03期

基金：

英国医学研究理事会;

关键词：

EPIGENETIC REGULATION; ACTIVE CHROMATIN; MAMMALIAN-CELLS; VARIANT H3.3; DNA; METHYLTRANSFERASES; RECOMBINATION; CHROMOSOMES; LENGTH;

D O I：

10.1101/gr.084947.108

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Little is known about the telomere chromatin dynamics of embryonic stem (ES) cell. Here, we demonstrate localization of histone H3.3 at interphase telomeres and enrichment of Ser31-phosphorylated H3.3 at metaphase telomeres in pluripotent mouse ES cells. Upon differentiation, telomeric H3.3S31P signal decreases, accompanied by increased association of heterochromatin repressive marks and decreased micrococcal nuclease sensitivity at the telomeres. H3.3 is recruited to the telomeres at late S/G2 phase, coinciding with telomere replication and processing. RNAi-depletion of H3.3 induces telomere-dysfunction phenotype, providing evidence for a role of H3.3 in the regulation of telomere chromatin integrity in ES cells. The distinctive changes in H3.3 distribution suggests the existence of a unique and functionally essential telomere chromatin in ES cells that undergoes dynamic differentiation-dependent remodeling during the process of differentiation.

引用

页码：404 / 414

页数：11

共 19 条

[1] The histone variant H3.3 marks active chromatin by replication-independent nucleosome assembly [J].