Rapid and simple detection of food poisoning bacteria by bead assay with a microfluidic chip-based system

被引：34

作者：

Ikeda, Masafumi ^{[1
]}

Yamaguchi, Nobuyasu ^{[1
]}

Tani, Katsuji ^{[1
]}

Nasu, Masao ^{[1
]}

机构：

[1] Osaka Univ, Grad Sch Pharmaceut Sci, Suita, Osaka 5650871, Japan

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2006年 / 67卷 / 02期

基金：

日本学术振兴会;

关键词：

bead assay; microfluidic chip-based system; pathogenic bacteria; rRNA-targeted probe;

D O I：

10.1016/j.mimet.2006.03.014

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A rapid bead assay for detecting pathogenic bacteria with a simple microfluidic chip-based system was developed. Five oligonucleotide probes corresponding to the 16S rRNA of the targeted bacteria were coupled covalently to fluorescent beads. Four species of bacteria (Escherichia coli, Salmonella euterica subsp. enterica serovar Enteritidis, Yersinia enterocolitica, and Bacillus cereus) were used as representative food-borne pathogenic bacteria. The RNAs extracted from pure cultures of these microorganisms were fluorescently labeled and hybridized to the oligonucleotide probes-immobilized fluorescent beads (Bead assay). The duplexes of RNAs and the probes-immobilized beads were analyzed with the commercially available microfluidic chip-based system. This bead assay provided results within 3 h following RNA extraction from bacterial cells. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：241 / 247

页数：7

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