Evolutionary dynamics of viral attenuation

被引:43
作者
Badgett, MR
Auer, A
Carmichael, LE
Parrish, CR
Bull, JJ [1 ]
机构
[1] Univ Texas, Sect Integrat Biol, Austin, TX 78712 USA
[2] Univ Texas, Inst Cellular & Mol Biol, Austin, TX 78712 USA
[3] Cornell Univ, Coll Vet Med, James A Baker Inst, Ithaca, NY 14853 USA
关键词
D O I
10.1128/JVI.76.20.10524-10529.2002
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The genetic trajectory leading to viral attenuation was studied in a canine parvovirus (CPV) strain grown on dog kidney cells for 115 transfers. Consensus sequences of viral populations at passages 0, 3, 30, 50, 80, and 115 were obtained from PCR products covering 86% of the genome; clones from each of the 80th and 115th passages were also sequenced, covering 69% of the genome. Sixteen changes were fixed in the 115th-passage virus sample. Levels of polymorphism were strikingly different over time, in part because of a plaque-cloning step at passage 112 that reduced variation: passage 80 had 19 variants common among the clones, but passage 115 had only a single common variant. Several mutations increased in the culture at the same time, with most reaching fixation only after the 80th passage. The pattern of evolution was consistent with recombination and not with separate selective sweeps of individual mutations. Thirteen of the changes observed were identical to or at the same positions as changes observed in other isolates of CPV or feline panleukopenia virus.
引用
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页码:10524 / 10529
页数:6
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