Automated carbohydrate profiling by capillary electrophoresis: A bioindustrial approach

被引：14

作者：

Khandurina, J ^{[1
]}

Blum, DL ^{[1
]}

Stege, JT ^{[1
]}

Guttman, A ^{[1
]}

机构：

[1] Diversa Corp, San Diego, CA 92121 USA

来源：

ELECTROPHORESIS | 2004年 / 25卷 / 14期

关键词：

biotechnology; carbohydrate profiling; high-performance capillary electrophoresis; laser-induced fluorescence; lignocellulosic biomass degradation;

D O I：

10.1002/elps.200305917

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Automated, high-resolution, quantitative, high-throughput analysis of mono- and oligosaccharides, produced by enzymatic digestion of cellohexaose (model substrate) and lignocellulosic biomass, is demonstrated using high-performance capillary electrophoresis in conjunction with a single-step fluorophore labeling strategy for sensitive laser-induced fluorescence detection. Unattended batch sample processing from 96-well plates enabled reliable industrial-scale carbohydrate analysis. Excellent resolution of mono- and oligosaccharides was achieved under suppressed electroosmotic flow conditions, using either covalently or dynamically coated fused-silica capillary columns. The proposed approach readily supports the demands of bioindustrial operation environments with respect to high-throughput carbohydrate profiling.

引用

页码：2326 / 2331

页数：6

共 21 条

[1]

[Anonymous], 1997, Techniques in Glycobiology

[2]

[Anonymous], 2002, CARBOHYDRATE ANAL MO

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