Determination of caffeine in tea samples by Fourier transform infrared spectrometry

被引：32

作者：

Ohnsmann, J ^{[1
]}

Quintás, G ^{[1
]}

Garrigues, S ^{[1
]}

de la Guardia, M ^{[1
]}

机构：

[1] Univ Valencia, Dept Analyt Chem, Edifici Jeroni Munoiz, Valencia 36100, Spain

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2002年 / 374卷 / 03期

关键词：

caffeine; tea; FTIR; solvent extraction;

D O I：

10.1007/s00216-002-1503-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A sustainable and environmentally friendly procedure has been developed for the FTIR determination of caffeine in tea leaf samples. The method is based on the extraction with ammonia and CHCl3 and direct determination of caffeine on the chloroform extracts using peak height absorbance measurements at 1658.5 cm(-1) and external calibration. The method provides a sensitivity of 0.2142 absorbance units mg(-1) mL and a limit of detection of 1 mg L-1, corresponding to 0.002% m/m caffeine in tea leaves. As compared with a reference procedure, based on UV absorbance measurement at 276 nm after low pressure column chromatography, the developed procedure reduces the consume of CHCl3 by a factor of 10, that of NH4OH by a factor of 20 and avoids the use of diethyl ether and Celite. The time required to do the analysis of a sample is 15 minutes as compared with the 6 hours for the reference one.

引用

页码：561 / 565

页数：5

共 28 条

[1] Validation of an isocratic HPLC method based on the use of ABZ+ plus phase for the simultaneous determination of methylxantines, chlorogenic acid, some hydroxy-benzoic and hydroxy-cinnamic acids. application to cocoa, coffee, tea and cola-drinks
Arlorio, M
Coïsson, JD
Martelli, A
[J]. CHROMATOGRAPHIA, 2000, 52 (9-10) : 579 - 583
[2] Flow injection Fourier transform infrared determination of caffeine in coffee
Bouhsain, Z
Garrigues, JM
Garrigues, S
de la Guardia, M
[J]. VIBRATIONAL SPECTROSCOPY, 1999, 21 (1-2) : 143 - 150
[3] CAPEDA A, 1990, ALIMENTARIA, V211, P23
[4] CAPEDA A, 1991, AN BROMATOL, V42, P241
[5] Simultaneous determination of caffeine, theobromine and theophylline in foods and pharmaceutical preparations by using ion chromatography
Chen, Q
Mou, S
Hou, X
Ni, Z
[J]. ANALYTICA CHIMICA ACTA, 1998, 371 (2-3) : 287 - 296
[6] Flow injection Fourier transform infrared determination of caffeine in soft drinks
Daghbouche, Y
Garrigues, S
Vidal, MT
delaGuardia, M
[J]. ANALYTICAL CHEMISTRY, 1997, 69 (06) : 1086 - 1091
[7] De Camargo MCR, 1999, J SCI FOOD AGR, V79, P1861, DOI 10.1002/(SICI)1097-0010(199910)79:13&lt
[8] 1861::AID-JSFA448&gt
[9] 3.0.CO
[10] 2-Y

← 1 2 3 →