Cell Sorting: Automated Separation of Mammalian Cells as a Function of Intracellular Fluorescence

被引：265

作者：

Hulett, H. R. ^{[1
]}

Bonner, W. A. ^{[1
]}

Barrett, Janet ^{[1
]}

Herzenberg, Leonard A. ^{[1
]}

机构：

[1] Stanford Univ, Sch Med, Dept Genet, Stanford, CA 94305 USA

来源：

JOURNAL OF IMMUNOLOGY | 2014年 / 193卷 / 05期

关键词：

D O I：

10.1126/science.166.3906.747

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A system for high-speed sorting of fluorescent cells was able to sort mouse spleen cells from Chinese hamster ovarian cells after development of fluorochromasia. Highly fluorescent fractions separated after similar treatment from mouse spleen cells immunized to sheep erythrocytes were enriched in antibody-producing cells by factors of 4 to 10.

引用

页码：747 / 749

页数：3

共 6 条

[1] ELECTRONIC SEPARATION OF BIOLOGICAL CELLS BY VOLUME [J].

FULWYLER, MJ .

SCIENCE, 1965, 150 (3698) :910-&

[2]

Jerne N. K., 1963, J EXP MED

[3]

Kamentsky L. A., SCIENCE, P630

[4] MEMBRANE PROPERTIES OF LIVING MAMMALIAN CELLS AS STUDIED BY ENZYMATIC HYDROLYSIS OF FLUOROGENIC ESTERS [J].

ROTMAN, B ;

PAPERMASTER, BW .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1966, 55 (01) :134-+

[5]

Sweet R. O., 1964, STANFORD UNIV TECH R

[6] CELL MICROFLUOROMETRY - A METHOD FOR RAPID FLUORESCENCE MEASUREMENT [J].

VANDILLA, MA ;

TRUJILLO, TT ;

MULLANEY, PF ;

COULTER, JR .

SCIENCE, 1969, 163 (3872) :1213-&

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