Morphine determination by HPLC with improved chemiluminescence detection using a conventional silica based column

被引：14

作者：

Amiott, E

Andrews, ARJ

机构：

[1] Department of Chemistry, Ohio University, Athens

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES | 1997年 / 20卷 / 02期

关键词：

D O I：

10.1080/10826079708010655

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new high performance liquid chromatography-chemiluminescence analysis system for morphine is described. This system has an on-column limit of detection of 20 pg for morphine, allowing morphine determination down to 1 ng/mL, with a 20 mu L injection size, without any concentration during sample preparation. Simultaneous determination of monoacetylmorphine to 15 ng/mL (300 pg on-column) is also achieved. The internal standard for both compounds is nalorphine and total analysis time is under 5 minutes.

引用

页码：311 / 325

页数：15

共 14 条

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