Effect of Hypoxia on the Calcium and Magnesium Content, Lipid Peroxidation Level, and Ca2+-ATPase Activity of Syncytiotrophoblast Plasma Membranes from Placental Explants

被引:25
作者
Chiarello, Delia I. [1 ]
Marin, Reinaldo [1 ]
Proverbio, Fulgencio [1 ]
Benzo, Zully [2 ]
Pinero, Sandy [1 ]
Botana, Desiree [1 ]
Abad, Cilia [1 ]
机构
[1] Inst Venezolano Invest Cient, Ctr Biofis & Bioquim, Lab Bioenerget Celular, Caracas 1020A, Venezuela
[2] Inst Venezolano Invest Cient, Ctr Quim, Lab Quim Analit, Caracas 1020A, Venezuela
关键词
ADENOSINE-TRIPHOSPHATASE ACTIVITY; OXIDATIVE STRESS; BASAL MEMBRANES; CA-ATPASE; PREECLAMPSIA; PREGNANCY; TRANSPORT; SULFATE; WOMEN;
D O I
10.1155/2014/597357
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 [微生物学]; 090105 [作物生产系统与生态工程];
摘要
In the current study the possible relationship between the Ca2+/Mg2+ ratio of human syncytiotrophoblast plasma membranes and their lipid peroxidation and Ca2+-ATPase activity was determined. Syncytiotrophoblast plasma membranes of placental explants cultured under hypoxia increased their lipid peroxidation and Ca2+ content, diminished their Ca2+-ATPase activity, and kept their Mg2+ content unchanged. Membranes preincubated with different concentrations of Ca2+ increased their Ca2+ content without changes in their Mg2+ content. There is a direct relationship between Ca2+ content and lipid peroxidation of the membranes, as well as an inverse relationship between their Ca2+ content and Ca2+-ATPase activity. On the contrary, preincubation of membranes with different concentrations of Mg2+ showed a higher Mg2+ content without changing their lipid peroxidation and Ca2+-ATPase activity. Explants cultured under hypoxia in the presence of 4mM MgSO4 showed similar values of lipid peroxidation and Ca2+-ATPase activity of their membranes compared to those of explants cultured under normoxia. Increased Ca2+ content of the membranes by interacting with negatively charged phospholipids could result in destabilizing effects of the membrane structure, exposing hydrocarbon chains of fatty acids to the action of free radicals. Mg2+ might exert a stabilizing effect of the membranes, avoiding their exposure to free radicals.
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页数:9
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